SIZ1 Antibody

Code CSB-PA909789XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIZ1 Polyclonal antibody
Uniprot No.
Target Names
SIZ1
Alternative Names
SIZ1 antibody; ULL1 antibody; YDR409W antibody; E3 SUMO-protein ligase SIZ1 antibody; EC 2.3.2.- antibody; E3 SUMO-protein transferase SIZ2 antibody; SAP and Miz-finger domain-containing protein 1 antibody; Ubiquitin-like protein ligase 1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SIZ1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Acts as an E3 ligase mediating SUMO/Smt3 attachment to septins and PCNA. May be involved in chromosome maintenance.
Gene References into Functions
  1. data suggest that SUMO attachment negatively regulates Flp1 levels, which may partially account for the increased 2 microm copy number in the siz1Delta siz2Delta strain PMID: 15870299
  2. the SP-RING and PINIT of Ull1/Siz1 are core domains of the SUMO ligase, and the other domains are regulatory for protein stability and subcellular localization PMID: 16109721
  3. The SUMO-specific ligase Siz1 both stimulates the overall efficiency of sumoylation and selects the modification site on PCNA. PMID: 18155241
  4. NMR titration experiments showed that yeast Siz1 SAP domain uses alpha2-helix for DNA binding. PMID: 18831036
  5. defects in Rad6 postreplication repair pathway and Siz1/Srs2 homologous recombination suppression (HRS) pathway genes suppressed the increased gross chromosomal rearrangement rates seen in asf1 mutants PMID: 19635810
  6. Report structure of the Siz/PIAS SUMO E3 ligase Siz1 and determinants required for SUMO modification of PCNA. PMID: 19748360

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Subcellular Location
Cytoplasm. Nucleus. Bud neck.
Protein Families
PIAS family
Database Links

KEGG: sce:YDR409W

STRING: 4932.YDR409W

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