skp Antibody

Code CSB-PA365201XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) skp Polyclonal antibody
Uniprot No.
Target Names
skp
Alternative Names
skp antibody; hlpA antibody; ompH antibody; b0178 antibody; JW0173 antibody; Chaperone protein Skp antibody; DNA-binding 17 kDa protein antibody; Histone-like protein HLP-1 antibody; Seventeen kilodalton protein antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) skp protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Molecular chaperone that interacts specifically with outer membrane proteins, thus maintaining the solubility of early folding intermediates during passage through the periplasm. Required for the efficient release of OmpA from the inner membrane, the maintenance of its solubility in the periplasm, and, in association with lipopolysaccharide (LPS), for the efficient folding and insertion of OmpA into the outer membrane.
Gene References into Functions
  1. CpxQ combats toxicity at the inner membrane by downregulating the synthesis of the periplasmic chaperone Skp. PMID: 27048800
  2. Bioinformatic analysis of amino acid conservation, structural analysis of LPS-binding proteins, and MD simulations confirm the absence of a specific lipopolysaccharide binding site on Skp, and reveal a highly conserved salt-bridge network PMID: 25809264
  3. The global lifetime of the OmpX-Skp and tOmpA-Skp chaperone-substrate complex is seven orders of magnitude longer, emerging from the short local lifetimes by avidity. PMID: 24077225
  4. Evidence presented suggests that DegP/Skp function to rescue OMPs that fall off the SurA pathway. PMID: 17908933
  5. Seventeen kDa protein (Skp) interactions of Skp-OmpA complexes with lipopolysaccharide change the conformation of outer mebrane protein A (OmpA) in the Skp complex for facilitated insertion and folding into membranes. PMID: 19382746

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Subcellular Location
Periplasm.
Protein Families
Skp family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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