Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SKP1A Polyclonal antibody

Uniprot No.

Q39255

Target Names

SKP1A

Alternative Names

SKP1A antibody; ASK1 antibody; SKP1 antibody; UIP1 antibody; At1g75950 antibody; T4O12.17 antibody; SKP1-like protein 1A antibody; SKP1-like 1 antibody; UFO-binding protein 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana SKP1A protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Involved in ubiquitination and subsequent proteasomal degradation of target proteins. Together with CUL1, RBX1 and a F-box protein, it forms a SCF E3 ubiquitin ligase complex. The functional specificity of this complex depends on the type of F-box protein. In the SCF complex, it serves as an adapter that links the F-box protein to CUL1. SCF(UFO) is required for vegetative and floral organ development as well as for male gametogenesis. SCF(TIR1) is involved in auxin signaling pathway. SCF(COI1) regulates responses to jasmonates. SCF(EID1) and SCF(AFR) are implicated in phytochrome A light signaling. SCF(ADO1), SCF(ADO2), SCF(ADO3) are related to the circadian clock. SCF(ORE9) seems to be involved in senescence. SCF(EBF1/EBF2) may regulate ethylene signaling. Plays a role during embryogenesis and early postembryonic development, especially during cell elongation and division. Contributes to the correct chromosome segregation during tetrad formation.

Gene References into Functions

Arabidopsis SKP-like proteins (ASKs) can significantly improve soluble expression of F-box proteins and maintain their bioactivity. PMID: 28985004
How ASK1 might regulate protein stability and further downstream gene expression PMID: 26940208
ASK1 is predominately expressed from leptotene to pachytene, and negatively regulates recombination in Arabidopsis. PMID: 16283376
Through the ASK1-mediated proteolysis pathway, ASK1 proteins showed pleiotropic functions including photomorphogenesis, circadian oscillation, post-translation process, stress-responses and cell expansion or elongation. PMID: 16699544
These results raise the interesting possibility that ASK1 controls chromatin structure by targeting of either an early regulator of meiotic progression or possibly matrix attachment proteins for destruction. PMID: 16940350
study represents a thorough investigation showing that retroposition can play an important role in the evolution of a plant gene family whose members do not encode mobile elements PMID: 17470057

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Subcellular Location

Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, phragmoplast. Note=Associated to mitotic spindle and phragmoplasts during cell division.

Protein Families

SKP1 family

Tissue Specificity

Accumulates only in meristematic cells. Expressed in inflorescence, shoot and root apical meristems, as well as in developing organs such as gametocytes and seeds. Also detected in cortical layer and epidermis of roots, leaves, pith and vascular bundle of

Database Links

KEGG: ath:AT1G75950

STRING: 3702.AT1G75950.1

UniGene: At.23531

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SKP1A Antibody

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