SKP1B Antibody

Code CSB-PA861794XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SKP1B Polyclonal antibody
Uniprot No.
Target Names
SKP1B
Alternative Names
SKP1B antibody; ASK2 antibody; UIP2 antibody; At5g42190 antibody; MJC20.30 antibody; SKP1-like protein 1B antibody; SKP1-like 2 antibody; UFO-binding protein 2 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana SKP1B protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in ubiquitination and subsequent proteasomal degradation of target proteins. Together with CUL1, RBX1 and a F-box protein, it forms a SCF E3 ubiquitin ligase complex. The functional specificity of this complex depends on the type of F-box protein. In the SCF complex, it serves as an adapter that links the F-box protein to CUL1. SCF(UFO) is required for vegetative and floral organ development as well as for male gametogenesis. SCF(TIR1) is involved in auxin signaling pathway. SCF(COI1) regulates responses to jasmonates. SCF(EID1) and SCF(AFR) are implicated in phytochrome A light signaling. SCF(ADO1), SCF(ADO2), SCF(ADO3) are related to the circadian clock. SCF(ORE9) seems to be involved in senescence. SCF(EBF1/EBF2) may regulate ethylene signaling. Plays a role during embryogenesis and early postembryonic development, especially during cell elongation and division. Contributes to the correct chromosome segregation during tetrad formation.
Gene References into Functions
  1. ASK1 and ASK2, are required for Agrobacterium-mediated plant transformation. PMID: 22486382
  2. ASK1 or ASK2 overexpression could rescue or partially rescue the Abscisic acid (ABA)insensitivity of abi5-1 mutants, respectively. PMID: 22074111
Subcellular Location
Nucleus.
Protein Families
SKP1 family
Tissue Specificity
Expressed in tips, cortical layer and epidermis of roots. Detected in whole seedling, vascular tissues, pith and vascular bundle of young stem, leaves, inflorescence meristem, young floral buds and organ primordia, flowers, developing seeds and through th
Database Links

KEGG: ath:AT5G42190

STRING: 3702.AT5G42190.1

UniGene: At.23304

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