Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SLAC1 Polyclonal antibody

Uniprot No.

Q9LD83

Target Names

SLAC1

Alternative Names

SLAC1 antibody; CDI3 antibody; OZS1 antibody; RCD3 antibody; At1g12480 antibody; F5O11.23 antibody; T12C24.3 antibody; Guard cell S-type anion channel SLAC1 antibody; Protein CARBON DIOXIDE INSENSITIVE 3 antibody; Protein OZONE-SENSITIVE 1 antibody; Protein RADICAL-INDUCED CELL DEATH 3 antibody; Protein SLOW ANION CHANNEL-ASSOCIATED 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana SLAC1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Slow, weak voltage-dependent S-type anion efflux channel involved in maintenance of anion homeostasis. Cl(-) efflux through SLAC1 causes membrane depolarization, which activates outward-rectifying K1 channels, leading to KCl and water efflux to reduce turgor further and cause stomatal closure, that reduces water loss and promotes leaf turgor. Essential for stomatal closure in response to CO(2), abscisic acid (ABA), ozone O(3), light/dark transitions, humidity change, calcium ions, hydrogen peroxide H(2)O(2), reactive oxygen species (ROS), and nitric oxide. Binds to the highly selective inward-rectifying potassium channels KAT1 and AKT2, and inhibits their actvities. Functions as an essential negative regulator of inward potassium channels in guard cells. Essential for the efficient stomatal closure and opening in guard cells. Involved in the local and/or systemic stomatal responses (e.g. stomatal closure) to light stress.

Gene References into Functions

Surveying in the locus, we extrapolated that the SLAC1 gene, which is associated with stomatal closure, was likely responsible for the difference of drought tolerance. PMID: 25695335
SLAC1 is required for rapid stomatal closure in response to ozone, reduced air humidity and darkness. PMID: 23703845
The predictive power of a systems dynamic model in Arabidopsis, is demonstrated to explain the paradoxical suppression of channels that facilitate K(+) uptake, slowing stomatal opening, by mutation of the SLAC1 anion channel. PMID: 23090586
Guard cells with mutations in slac1 exhibit dramatically reduced inwardly-rectifying K+ channel currents. Decreasing the cytosolic Ca2+ concentration rapidly restores K+ channel currents in slac1 guard cells. PMID: 23126621
Data suggest that SLAC1 may be a pathway providing CO(2) for photosynthesis in the guard cells. PMID: 21931667
Guard cell anion channel SLAC1 is regulated by CDPK protein kinases with distinct Ca2+ affinities. PMID: 20385816
SLAC1 represents the slow, deactivating, weak voltage-dependent anion channel of guard cells controlled by phosphorylation/dephosphorylation PMID: 19955405
These study results suggested that OZS1 helps to close stomata, being not involved in the responses to light, abscisic acid, high concentrations of carbon dioxide and ozone. PMID: 18084014
The loss-of-function mutation in SLAC1 was accompanied by an over-accumulation of the osmoregulatory anions in guard cell protoplasts PMID: 18305482
The plasma membrane protein SLAC1 is essential for stomatal closure in response to CO2, abscisic acid, ozone, light/dark transitions, humidity change, calcium ions, hydrogen peroxide and nitric oxide PMID: 18305484

Hide All

Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

SLAC1 S-type anion channel family

Tissue Specificity

Preferentially expressed in guard cells. Also detected in the vascular strands close to the leaf margins.

Database Links

KEGG: ath:AT1G12480

STRING: 3702.AT1G12480.1

UniGene: At.48182

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SLAC1 Antibody

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