Product Details

Purity

>85% (SDS-PAGE)

Target Names

SLAC1

Uniprot No.

Q9LD83

Alternative Names

SLAC1; CDI3; OZS1; RCD3; At1g12480; F5O11.23; T12C24.3; Guard cell S-type anion channel SLAC1; Protein CARBON DIOXIDE INSENSITIVE 3; Protein OZONE-SENSITIVE 1; Protein RADICAL-INDUCED CELL DEATH 3; Protein SLOW ANION CHANNEL-ASSOCIATED 1

Species

Arabidopsis thaliana (Mouse-ear cress)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Slow, weak voltage-dependent S-type anion efflux channel involved in maintenance of anion homeostasis. Cl(-) efflux through SLAC1 causes membrane depolarization, which activates outward-rectifying K1 channels, leading to KCl and water efflux to reduce turgor further and cause stomatal closure, that reduces water loss and promotes leaf turgor. Essential for stomatal closure in response to CO(2), abscisic acid (ABA), ozone O(3), light/dark transitions, humidity change, calcium ions, hydrogen peroxide H(2)O(2), reactive oxygen species (ROS), and nitric oxide. Binds to the highly selective inward-rectifying potassium channels KAT1 and AKT2, and inhibits their actvities. Functions as an essential negative regulator of inward potassium channels in guard cells. Essential for the efficient stomatal closure and opening in guard cells. Involved in the local and/or systemic stomatal responses (e.g. stomatal closure) to light stress.

Gene References into Functions

Surveying in the locus, we extrapolated that the SLAC1 gene, which is associated with stomatal closure, was likely responsible for the difference of drought tolerance. PMID: 25695335
SLAC1 is required for rapid stomatal closure in response to ozone, reduced air humidity and darkness. PMID: 23703845
The predictive power of a systems dynamic model in Arabidopsis, is demonstrated to explain the paradoxical suppression of channels that facilitate K(+) uptake, slowing stomatal opening, by mutation of the SLAC1 anion channel. PMID: 23090586
Guard cells with mutations in slac1 exhibit dramatically reduced inwardly-rectifying K+ channel currents. Decreasing the cytosolic Ca2+ concentration rapidly restores K+ channel currents in slac1 guard cells. PMID: 23126621
Data suggest that SLAC1 may be a pathway providing CO(2) for photosynthesis in the guard cells. PMID: 21931667
Guard cell anion channel SLAC1 is regulated by CDPK protein kinases with distinct Ca2+ affinities. PMID: 20385816
SLAC1 represents the slow, deactivating, weak voltage-dependent anion channel of guard cells controlled by phosphorylation/dephosphorylation PMID: 19955405
These study results suggested that OZS1 helps to close stomata, being not involved in the responses to light, abscisic acid, high concentrations of carbon dioxide and ozone. PMID: 18084014
The loss-of-function mutation in SLAC1 was accompanied by an over-accumulation of the osmoregulatory anions in guard cell protoplasts PMID: 18305482
The plasma membrane protein SLAC1 is essential for stomatal closure in response to CO2, abscisic acid, ozone, light/dark transitions, humidity change, calcium ions, hydrogen peroxide and nitric oxide PMID: 18305484

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Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

SLAC1 S-type anion channel family

Tissue Specificity

Preferentially expressed in guard cells. Also detected in the vascular strands close to the leaf margins.

Database Links

KEGG: ath:AT1G12480

STRING: 3702.AT1G12480.1

UniGene: At.48182

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Recombinant Arabidopsis thaliana Guard cell S-type anion channel SLAC1 (SLAC1), partial

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