SLG1 Antibody

Code CSB-PA345426XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SLG1 Polyclonal antibody
Uniprot No.
Target Names
SLG1
Alternative Names
SLG1 antibody; WSC1 antibody; YOR008C antibody; UNF378 antibody; Protein SLG1 antibody; Cell wall integrity and stress response component 1 antibody; Synthetic lethal with GAP protein 1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SLG1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Plays a role during G1 to regulate entering or exiting the cell cycle. Involved in stress responses. Has a role in cell wall integrity signaling. Activates ROM1 or ROM2 catalyzed guanine nucleotide exchange toward RHO1. Important regulator of the actin cytoskeleton rearrangements in conditions of cell wall expansion and membrane stretching. Specifically required for the actin reorganization induced by hypo-osmotic shock. Multicopy suppressor of 1,3-beta-glucan synthase (GS). Activates GS upstream of RHO1. Acts positively on the PKC1-MAPK pathway. Activates transiently SLT2 during alkaline stress, which leads to an increase in the expression of several specific genes.
Gene References into Functions
  1. wsc1 mutation disrupted biofilm formation in a Flo11p-independent manner. PMID: 24318926
  2. Study indicates an important role of the extracellular domain of Wsc1 in mediating signal specificity of this sensor to detect cell wall damage. PMID: 20958245
  3. Data show that Wsc1 uses a clathrin-dependent NPFDD signal, Wsc2 relies on a specific lysine residue (K495). PMID: 20652590
  4. endoplasmic reticulum surveillance evasion makes Wsc1p an obligate substrate of Golgi quality control PMID: 20130083
  5. WSC1 is involved in reorganization of the actin cytoskeleton in response to hypo-osmotic shock in Saccharomyces cerevisiae. PMID: 15484288
  6. Participates in the regulation of a Rho3/4-dependent cellular mechanism, and this is distinct from the role of Wsc1p in the PKC-MAP kinase pathway. PMID: 16514150
  7. The dynamic localization of Wsc1p, a sensor of the extracellular wall in yeast, resembles polarized distribution of certain extracellular matrix-sensing integrins through endocytic recycling. PMID: 17065552
  8. Chimers of Wsc1 fused to GFP have been employed to show that intracellular and membrane distribution is only dependent on a membrane-anchored cytoplasmic tail. PMID: 17761172

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Subcellular Location
Cell membrane; Single-pass type I membrane protein.
Database Links

KEGG: sce:YOR008C

STRING: 4932.YOR008C

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