Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SLI15 Polyclonal antibody

Uniprot No.

P38283

Target Names

SLI15

Alternative Names

SLI15 antibody; YBR156C antibody; YBR1206 antibody; Inner centromere protein-related protein SLI15 antibody; INCENP-related protein SLI15 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SLI15 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Required for high-fidelity chromosome segregation during the later part of each cell cycle in association with the protein kinase IPL1. Stimulates IPL1 kinase activity and facilitates its association with the mitotic spindle. Has a role in attaching the kinetochores to the microtubules and ensuring that sister kinetochores connect to opposite poles.

Gene References into Functions

Ipl1-dependent phosphorylation of Sli15 therefore inhibits its association with microtubules both in vivo and in vitro and may negatively regulate the tension checkpoint mechanism. PMID: 24558497
phosphorylation of INCENP/Sli15 is required for activation of the kinase Aurora B/Ipl1 and can occur in trans. PMID: 23814063
an engineered truncation of the Sli15 subunit of budding yeast chromosomal passenger complex that eliminates association with the inner centromere nevertheless supports proper chromosome segregation during both mitosis and meiosis PMID: 23604256
Cdk1 and Ipl1/Aurora cooperatively modulate microtubule dynamics and that Ipl1/Aurora-dependent phosphorylation of Sli15 controls spindle function by excluding the CPC from spindle regions engaged in microtubule polymerization PMID: 21727193
dephosphorylation by Cdc14 inactivates the mitotic checkpoint PMID: 20619650
Using an in vitro approach based on the sequence-specific budding yeast centromere, a complex of the chromosomal passenger proteins Bir1 and Sli15 (Survivin and INCENP) that links centromeres to microtubules was identified. PMID: 17174893
The interaction between Bir1p and Sli15p is specifically involved in regulating septin dynamics during anaphase. PMID: 17652458
Findings reveal a new role for the central kinetochore in promoting Sli15 function, and suggest functional Ame1 is required for the correction of defective attachments by promoting the localization of Sli15/INCENP at kinetochores. PMID: 19597337

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Subcellular Location

Nucleus. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore.

Protein Families

INCENP family

Database Links

KEGG: sce:YBR156C

STRING: 4932.YBR156C

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SLI15 Antibody

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