SLM1 Antibody

Code CSB-PA328123XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SLM1 Polyclonal antibody
Uniprot No.
Target Names
SLM1
Alternative Names
SLM1 antibody; LIT2 antibody; YIL105C antibody; Phosphatidylinositol 4,5-bisphosphate-binding protein SLM1 antibody; Synthetic lethal with MSS4 protein 1 antibody; TORC2 effector protein SLM1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SLM1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Together with SLM2, effector of the TORC2- and calcineurin-signaling pathways. Phosphorylated and activated by TORC2 under favorable growth conditions. Mediates actin polarization via inhibition of calcineurin-dependent transcription. Upon nutrient limitation or environmental stress, gets dephosphorylated by calcineurin. Dephosphorylation inhibits its interaction with TORC2, thereby antagonizing TORC2 signaling and mediating calcineurin-dependent actin depolarization. Also functions in heat-induced, calcineurin-mediated uracil permease (FUR4) endocytosis.
Gene References into Functions
  1. Slm1/Slm2 and TORC2-dependent phosphorylation of Ypk1 is coupled to the biosynthesis of complex sphingolipids and to their levels within the plasma membrane. PMID: 22895050
  2. Slm proteins are required for proper eisosome organization, endocytic trafficking and recycling in the yeast Saccharomyces cerevisiae. PMID: 21451250
  3. Slm1 and Slm2 integrate inputs from the Phosphatidylinositol-4,5-bisphosphate and TORC2 to modulate polarized actin assembly and growth PMID: 15689497
  4. calcineurin may modulate sphingolipid-dependent events through regulation of Slm1 and Slm2 PMID: 16738335
  5. Data suggest that Slm1 and Slm2 define a molecular link between phosphoinositide and sphingolipid signaling and thereby regulate actin cytoskeleton organization. PMID: 16847337
  6. Slm proteins are subject to regulation by multiple signals, including PI4,5P(2), TORC2, and sphingolipids, and may thus integrate inputs from different signaling pathways to temporally and spatially control actin polarization PMID: 17101780

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Subcellular Location
Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Localizes to cortical punctate structures. Correct localization requires phosphatidylinositol 4,5-bisphosphate and functional TORC2.
Database Links

KEGG: sce:YIL105C

STRING: 4932.YIL105C

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