Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SLX4 Polyclonal antibody

Uniprot No.

Q12098

Target Names

SLX4

Alternative Names

SLX4 antibody; YLR135W antibody; L3140 antibody; Structure-specific endonuclease subunit SLX4 antibody; Synthetic lethal of unknown function protein 4 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SLX4 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Regulatory subunit that interacts with and increases the activity of different structure-specific endonucleases. Has several distinct roles in protecting genome stability by resolving diverse forms of deleterious DNA structures. Component of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for simple Y, 5'-flap and replication fork-like structures. It cleaves the strand bearing the 5'-non-homologous arm at the branch site junction and generates ligatable, nicked products from the 5'-flap or replication fork substrates. Plays a critical role in maintaining the integrity of the ribosomal DNA (rDNA) loci, where it has a role in re-starting stalled replication forks. Has Holliday junction resolvase activity in vitro. Interacts with the structure-specific RAD1-RAD10 endonuclease and promotes RAD1-RAD10-dependent 3'-non-homologous tail removal (NHTR) during repair of double-strand breaks by single-strand annealing. SLX4 also promotes recovery from DNA-alkylation-induced replisome stalling during DNA replication by facilitating the error-free mode of lesion bypass. This does not require SLX1 or RAD1-RAD10, but probably RTT107.

Gene References into Functions

SLX4 contributes to the appropriate distribution of crossovers and meiotic double-strand break formation on bivalents during meiosis. PMID: 27172214
proper termination of DNA damage checkpoint signaling following the bypass of replication blocks imposed by alkylated DNA adducts requires the concerted and highly complementary actions of Slx4 and the PP4 phosphatase PMID: 26362319
Slx4 and Rtt107 control checkpoint signalling and DNA resection at double-strand breaks. PMID: 26490958
Rtt107 is a multi-functional scaffold supporting replication progression with partners SUMO, ubiquitin ligase E3, and SLX4. PMID: 26439300
The Slx4-Dpb11 scaffold complex coordinates the response to replication fork stalling in S-phase and the subsequent mitosis. PMID: 25496009
Authors demonstrate that Slx4 is recruited to chromatin behind stressed replication forks, in a region that is spatially distinct from that occupied by the replication machinery. PMID: 26113155
Multiple fork repair factors associate with Rtt107 or Slx4, supporting that Mec1-dependent assembly of the Rtt107-Slx4-Dpb11 complex functions to coordinate fork repair. PMID: 20670896
Mre11 (with Sae2) and Slx4 are structure-specific endonucleases that protect cells from trapped topoisomerase by removing topoisomerase-DNA adducts PMID: 15834151
The phosphorylation of Slx4 after DNA damage by multiple genotoxic agents, and its relationship to Mec1 and Tel1 gene expression are reported. PMID: 15975089
Rtt107 binds to the DNA repair protein Slx4. Cells lacking Slx4 have similar phenotypes to those lacking Rtt107. Slx4 is required for the DNA damage dependent phosphorylation of Rtt107 by the checkpoint kinase Mec1. PMID: 16267268
Phosphorylation of SLX4 by MEC1 and TEL1 regulates the single-strand annealing mode of DNA repair in budding yeast. PMID: 17636031
Slx4 is a known target of the DNA damage response that forms a complex with Rad1-Rad10 and is critical for 3'-end processing during repair of DSBs by single-strand annealing PMID: 18579504

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Subcellular Location

Nucleus. Cytoplasm.

Protein Families

SLX4 family

Database Links

KEGG: sce:YLR135W

STRING: 4932.YLR135W

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SLX4 Antibody

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