Recombinant Saccharomyces cerevisiae Structure-specific endonuclease subunit SLX4 (SLX4), partial

Code CSB-YP612282SVG
MSDS
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Source Yeast
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Code CSB-EP612282SVG
MSDS
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Source E.coli
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Code CSB-EP612282SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP612282SVG
MSDS
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Source Baculovirus
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Code CSB-MP612282SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SLX4
Uniprot No.
Alternative Names
SLX4; YLR135W; L3140; Structure-specific endonuclease subunit SLX4; Synthetic lethal of unknown function protein 4
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Regulatory subunit that interacts with and increases the activity of different structure-specific endonucleases. Has several distinct roles in protecting genome stability by resolving diverse forms of deleterious DNA structures. Component of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for simple Y, 5'-flap and replication fork-like structures. It cleaves the strand bearing the 5'-non-homologous arm at the branch site junction and generates ligatable, nicked products from the 5'-flap or replication fork substrates. Plays a critical role in maintaining the integrity of the ribosomal DNA (rDNA) loci, where it has a role in re-starting stalled replication forks. Has Holliday junction resolvase activity in vitro. Interacts with the structure-specific RAD1-RAD10 endonuclease and promotes RAD1-RAD10-dependent 3'-non-homologous tail removal (NHTR) during repair of double-strand breaks by single-strand annealing. SLX4 also promotes recovery from DNA-alkylation-induced replisome stalling during DNA replication by facilitating the error-free mode of lesion bypass. This does not require SLX1 or RAD1-RAD10, but probably RTT107.
Gene References into Functions
  1. SLX4 contributes to the appropriate distribution of crossovers and meiotic double-strand break formation on bivalents during meiosis. PMID: 27172214
  2. proper termination of DNA damage checkpoint signaling following the bypass of replication blocks imposed by alkylated DNA adducts requires the concerted and highly complementary actions of Slx4 and the PP4 phosphatase PMID: 26362319
  3. Slx4 and Rtt107 control checkpoint signalling and DNA resection at double-strand breaks. PMID: 26490958
  4. Rtt107 is a multi-functional scaffold supporting replication progression with partners SUMO, ubiquitin ligase E3, and SLX4. PMID: 26439300
  5. The Slx4-Dpb11 scaffold complex coordinates the response to replication fork stalling in S-phase and the subsequent mitosis. PMID: 25496009
  6. Authors demonstrate that Slx4 is recruited to chromatin behind stressed replication forks, in a region that is spatially distinct from that occupied by the replication machinery. PMID: 26113155
  7. Multiple fork repair factors associate with Rtt107 or Slx4, supporting that Mec1-dependent assembly of the Rtt107-Slx4-Dpb11 complex functions to coordinate fork repair. PMID: 20670896
  8. Mre11 (with Sae2) and Slx4 are structure-specific endonucleases that protect cells from trapped topoisomerase by removing topoisomerase-DNA adducts PMID: 15834151
  9. The phosphorylation of Slx4 after DNA damage by multiple genotoxic agents, and its relationship to Mec1 and Tel1 gene expression are reported. PMID: 15975089
  10. Rtt107 binds to the DNA repair protein Slx4. Cells lacking Slx4 have similar phenotypes to those lacking Rtt107. Slx4 is required for the DNA damage dependent phosphorylation of Rtt107 by the checkpoint kinase Mec1. PMID: 16267268
  11. Phosphorylation of SLX4 by MEC1 and TEL1 regulates the single-strand annealing mode of DNA repair in budding yeast. PMID: 17636031
  12. Slx4 is a known target of the DNA damage response that forms a complex with Rad1-Rad10 and is critical for 3'-end processing during repair of DSBs by single-strand annealing PMID: 18579504

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Subcellular Location
Nucleus. Cytoplasm.
Protein Families
SLX4 family
Database Links

KEGG: sce:YLR135W

STRING: 4932.YLR135W

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