Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SLX5 Polyclonal antibody

Uniprot No.

P32828

Target Names

SLX5

Alternative Names

SLX5 antibody; HEX3 antibody; YDL013W antibody; D2875 antibody; E3 ubiquitin-protein ligase complex SLX5-SLX8 subunit SLX5 antibody; EC 2.3.2.27 antibody; Hexose metabolism-related protein HEX3 antibody; RING-type E3 ubiquitin transferase SLX5 antibody; Synthetic lethal of unknown function protein 5 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SLX5 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Component of the SUMO-targeted ubiquitin ligase (STUbL) complex SLX5/SLX8 that mediates ubiquitination and subsequent desumoylation of sumoylated proteins and proteins containing SUMO-like domains for their degradation. The STUbL complex SLX5/SLX8 stimulates ubiquitin conjugating enzymes, including UBC1, UBC4, UBC5 and UBC13-MMS2, and mediates the proteolytic down-regulation of sumoylated proteins. The STUbL complex SLX5/SLX8 is involved in ubiquitin-mediated degradation of histone variant CSE4, preventing mislocalization to euchromatin. The complex plays an essential role in maintenance of chromosome stability and links SUMO-dependent ubiquitination to a centromere-specific function during mitosis. The complex is involved in proteolysis of spindle positioning protein KAR9 and ensures correct spindle function by regulating levels of microtubule-associated proteins. During replication, the complex helps prevent DNA lesions via recombination and has a role in localizing the DNA damage protein DCD2. The complex especially ubiquitinates the nuclease YEN1 and prevents persistent accumulation of a fraction of YEN1 associated with sites of activity in late G2/M and helps maintain the balance between pro- and anti-crossover pathways during homologous recombination. It is also involved in ubiquitin-mediated degradation of DNA repair proteins RAD52 and RAD57. Along with SIR2, promotes silencing of genes at telomeric or ribosomal DNA (rDNA) loci. Finally, the complex is recruited to distinct genomic hotspots of non-H2B protein ubiquitination (ub-hotspots) by the sumoylated transcription factor-like protein EUC1 where it ubiquitinates EUC1 and presumably other targets.

Gene References into Functions

SLX5 suppressor mutations of tdp1 wss1 synthetic lethality created new lysine residues in a 398 amino acid domain that is completely free of lysine. They result in the auto-ubiquitination and partial proteolysis of Slx5. PMID: 28550017
RecQ-like helicase Sgs1 subcellular localization is regulated by SUMO-targeted ubiquitin ligase Slx5-Slx8 in response to DNA damage, presumably to prevent illegitimate recombination events. PMID: 25588990
Siz1 can be ubiquitylated in vivo and is degraded in an Slx5-dependent manner. PMID: 24196836
distinct SUMO ligases work in concert with Esc2 and Slx5-Slx8 to control substrate specificity and sumoylation homeostasis to prevent GCRs. PMID: 23935535
Component or regulator of the small ubiquitin-related modifier (SUMO) protein pathway. PMID: 16387868
Data suggest that, during replication, the Slx5-Slx8 complex helps prevent DNA lesions that are acted upon by recombination, and inhibits Rad51-independent recombination via modulating the sumoylation of DNA repair proteins. PMID: 17591698
the Slx5 and slx8 complex is capable of interacting directly with the SUMO pathway PMID: 17669696
the Hex3.Slx8 complex has a robust substrate-specific E3 ubiquitin ligase activity. PMID: 17848550
The Slx5-Slx8 complex, but not its individual units, stimulates several ubiquitin ligase conjugating enzymes. PMID: 18032921
Slx5-Slx8 ubiquitin ligase is stimulated by poly-SUMO conjugates and that it can ubiquitinate a poly-small ubiquitin-like modifier chain PMID: 18499666
study shows a nuclear pore subcomplex and Slx5/Slx8, a SUMO-dependent ubiquitin ligase physically interact; findings suggest that DNA strand breaks are shunted to nuclear pores for a repair pathway controlled by a conserved SUMO-dependent E3 ligase PMID: 18948542
the DNA-damage targeting of Slx5/Slx8 depends on formation of the heterodimer and that this occurs at a subset of nuclear foci also containing DNA damage repair and checkpoint factors. PMID: 19270524

Hide All

Subcellular Location

Nucleus. Chromosome. Chromosome, centromere, kinetochore. Chromosome, centromere.

Database Links

KEGG: sce:YDL013W

STRING: 4932.YDL013W

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SLX5 Antibody

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