Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) slyD Polyclonal antibody

Uniprot No.

P0A9K9

Target Names

slyD

Alternative Names

slyD antibody; b3349 antibody; JW3311 antibody; FKBP-type peptidyl-prolyl cis-trans isomerase SlyD antibody; PPIase antibody; EC 5.2.1.8 antibody; Histidine-rich protein antibody; Metallochaperone SlyD antibody; Rotamase antibody; Sensitivity to lysis protein D antibody; WHP antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) slyD protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Folding helper with both chaperone and peptidyl-prolyl cis-trans isomerase (PPIase) activities. Chaperone activity prevents aggregation of unfolded or partially folded proteins and promotes their correct folding. PPIases catalyze the cis-trans isomerization of Xaa-Pro bonds of peptides, which accelerates slow steps of protein folding and thus shortens the lifetime of intermediates. Both strategies lower the concentration of intermediates and increase the productivity and yield of the folding reaction. SlyD could be involved in Tat-dependent translocation, by binding to the Tat-type signal of folded proteins. The PPIase substrate specificity, carried out with synthetic peptides of the 'suc-Ala-Xaa-Pro-Phe-4NA' type (where Xaa is the AA tested), was found to be Phe > Ala > Leu.; Required for lysis of phiX174 infected cells by stabilizing the hydrophobic viral lysis protein E and allowing it to accumulate to the levels required to exert its lytic effect. May act by a chaperone-like mechanism.; Also involved in hydrogenase metallocenter assembly, probably by participating in the nickel insertion step. This function in hydrogenase biosynthesis requires chaperone activity and the presence of the metal-binding domain, but not PPIase activity.

Gene References into Functions

SlyD has an essential role in the generation of the fully cofactor-equipped hydrogenase large subunits in the stationary phase where the level of each Hyd enzyme is finely tuned by SlyD for optimal enzyme activity PMID: 25620052
the importance of the unusual metal-binding domain of E. coli SlyD in hydrogenase biogenesis PMID: 22310044
the metal selectivity of SlyD PMID: 22047179
On the basis of the presented data a model for nickel binding to SlyD as well as its role in nickel homeostasis is discussed. PMID: 19947632
SlyD has a role in the Escherichia coli hydrogenase biosynthetic pathway PMID: 15569666
SlyD FKBP accelerated the aggregation of human alpha-synuclein in vitro PMID: 16410343
lysis protein E from bacteriophage phiX174 was found to be a potent inhibitor of SlyD-catalysed peptidyl-prolyl isomerization (IC(50) 0.15 microM), implying a strong interaction between E of PhiX174 and SlyD PMID: 17005977
Data show that insertion of a chaperone domain from E. coli SlyD converts human FKBP12 into a powerful catalyst of protein folding. PMID: 17397867

Hide All

Subcellular Location

Cytoplasm.

Protein Families

FKBP-type PPIase family

Database Links

KEGG: ecj:JW3311

STRING: 316385.ECDH10B_3524

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-PA359315XA01ENV
Size	Enquire
Have Questions?	Leave a Message or Start an on-line Chat

slyD Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background