RAD5A Antibody

Code CSB-PA875517XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RAD5A Polyclonal antibody
Uniprot No.
Target Names
RAD5A
Alternative Names
RAD5A antibody; RAD5 antibody; At5g22750 antibody; MDJ22.17 antibody; DNA repair protein RAD5A antibody; EC 3.6.4.- antibody; Putative SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3-like 2 antibody; SMARCA3-like protein 2 antibody; RAD5 homolog A antibody; AtRAD5a antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana RAD5A protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Functions in error-free postreplication DNA repair or DNA-damage tolerance (DTT) pathway. Required for homologous recombination (HR) induced by DNA double-strand break (DSB) in somatic cells. Required for damage-induced DNA repair, independently of MUS81 and RECQL4A. Plays a role in synthesis-dependent strand annealing (SDSA) but not in single-strand annealing (SSA). Possesses double-stranded DNA-dependent ATPase activity. Is able to regress replication forks with preference for forks with a leading strand gap. Is able to catalyze branch migration of Holliday junctions and is unaffected by protein blockades.
Gene References into Functions
  1. The helicase domain as well as the RING domain are essential for the function of RAD5A in DNA repair. Further de fi ning the role of RAD5A in the complex network of DNA repair factors, we present evidence that RAD5A acts independently of various other well-known DNA repair pathways. PMID: 28509359
  2. suggesting that AtRAD5a and AtREV3 are required for error-free and translesion synthesis branches of DNA-damage tolerance, respectively. [RAD5a] PMID: 21549648
  3. Ubiquitin-conjugating enzymes and RAD5a proteins are involved in the ubiquitination of plant PCNA at lysine 164. PMID: 23314815
  4. RAD5A and RECQ4A act through two independent pathways for intrastrand cross-link DNA repair. PMID: 20971895
  5. RAD5-dependent error-free branch of postreplication repair is conserved between yeast and plants, and that AtRad5a might be functionally homologous to ScRad5 PMID: 18310306

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Subcellular Location
Nucleus.
Protein Families
SNF2/RAD54 helicase family, RAD16 subfamily
Database Links

KEGG: ath:AT5G22750

STRING: 3702.AT5G22750.1

UniGene: At.31042

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