SML1 Antibody

Code CSB-PA567731XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SML1 Polyclonal antibody
Uniprot No.
Target Names
SML1
Alternative Names
SML1 antibody; YML058W antibody; YM9958.04Ribonucleotide reductase inhibitor protein SML1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SML1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Strong inhibitor of ribonucleotide reductase (RNR1) and is involved in regulating dNTP production.
Gene References into Functions
  1. Multiple turnover kinetics show that Sml1 inhibition of dGTP/ADP- and ATP/CDP-bound ScRR follows a mixed inhibition mechanism. However, Sml1 cooperatively binds to the ES complex in the dGTP/ADP form, whereas with ATP/CDP, Sml1 binds weakly and noncooperatively PMID: 27155231
  2. curcumin induces degradation of Sml1p, a ribonucleotide reductase inhibitor involved in regulating dNTPs production. PMID: 23520547
  3. this Mec1-independent pathway converges with the Rad53/Dun1-regulated checkpoint kinase cascade and leads to the degradation of the ribonucleotide reductase inhibitor Sml1 PMID: 16287875
  4. Sml1 competes with RNR1 C-terminal domain for association with RNR1 N-terminal domain to hinder the accessibility of the CX2C motif to the active site for RNR1 regeneration. PMID: 17277086
  5. The amino(N)-terminal domain protects the carboxyl-terminal domain of Sml1 from degradation and may explain why the N-terminal region has been conserved in evolution. PMID: 19086274

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Subcellular Location
Nucleus. Cytoplasm.
Database Links

KEGG: sce:YML058W

STRING: 4932.YML058W

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