Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) Smn Polyclonal antibody

Uniprot No.

Q9VV74

Target Names

Smn

Alternative Names

Smn antibody; CG16725 antibody; Survival motor neuron protein antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) Smn protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm, is required for pre-mRNA splicing in the nucleus and acts as a chaperone that discriminates target and non-target RNAs of Sm proteins. Required for normal expression of spliceosomal snRNAs and for U12 intron splicing. Required in cholinergic neurons, but not in motor neurons, to ensure correct splicing and proper levels of stas mRNA and normal neurotransmitter release by motor neurons. However, Smn is required in motor neurons, but not in cholinergic neurons, for normal motor behavior but plays no role in synaptic transmission according to PubMed:23103409. In both muscle and neurons, required for the formation of a normal neuromuscular junction (NMJ) structure. Plays a neuron-specific role in long-term homeostatic compensation at the larval NMJ. In the thorax of adult flies, required for Act88F, an indirect flight muscle (IFM)-specific actin, expression and for proper IFM myofibril formation. In nurse cells, oocytes and follicle cells, required to maintain normal organization of nuclear compartments including chromosomes, nucleoli, Cajal bodies, histone locus bodies and heterochromatin. Required for the functional integrity of the cytoplasmic U snRNP body (U body) and P body. Required in dividing postembryonic neuroblasts (pNBs) for the correct basal localization of mira. The tight regulation of its expression is critical for stem cell division, proliferation and differentiation in male germline and developing central nervous system (CNS). Required for tracheal terminal cell lumen formation.

Gene References into Functions

Role of SMN in oogenesis: key requirement for mRNA trafficking. PMID: 29895323
Intragenic complementation analyses reveal the potential for dominant negative interactions between wild-type and mutant SMN subunits, highlighting the essential nature of the YG box in formation of higher-order SMN multimers. PMID: 25144193
Smn mutants displayed a prominent increase in levels of stress-responsive transcripts, indicating a systemic response to the developmental arrest induced by loss of SMN protein PMID: 24006466
A unique interactome for SMN provides a knowledge base for a better understanding of spinal muscular atrophy. PMID: 23757500
Our results uncover a hitherto under-appreciated role for SMN in homeostatic regulatory mechanisms at motor synapses. PMID: 23103409
SMN's role in small nuclear ribonucleoproteins biogenesis can be uncoupled from the organismal viability and locomotor defects. PMID: 22813737
Drosophila SMN mutants have reduced muscle size and defective locomotion, motor rhythm, and motor neuron neurotransmission to alter this phenotype, SMN must be expressed in proprioceptive neurons and interneurons in the motor circuit to nonautonomously correct defects in motor neurons and muscles. PMID: 23063130
Study shows that SMN deficiency perturbs splicing and decreases the expression of a subset of U12 intron-containing genes in mammalian cells and Drosophila larvae. PMID: 23063131
We conclude that highly regulated SMN levels are essential to drive timely cell proliferation and cell differentiation. PMID: 21490958
Smn activity regulates the expression of FGF signaling components and thus FGF signaling PMID: 21300852
dSMN is involved in signal pathways of apoptotic cell death in Drosophila PMID: 12783893
SMN is a sarcomeric protein and has a function in striated muscle. PMID: 17353360
SMN is required for the functional integrity of the U body-P body pathway, which in turn is important for maintaining proper nuclear architecture. PMID: 19464282

Hide All

Subcellular Location

Cytoplasm. Nucleus, gem. Cytoplasm, myofibril, sarcomere, I band. Cytoplasm, myofibril, sarcomere, Z line.

Protein Families

SMN family

Tissue Specificity

In late first instar larvae, expressed in pNBs. Expression increases as the pNBs enlarge, with the highest accumulation observed in dividing pNBs of second and third instar larvae. Enriched in type ID (thoracic and brain lobe), type IA and all the mira-ex

Database Links

KEGG: dme:Dmel_CG16725

STRING: 7227.FBpp0302954

UniGene: Dm.2679

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Smn Antibody

Product Details

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Target Background