smu-1 Antibody

Code CSB-PA133246XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans smu-1 Polyclonal antibody
Uniprot No.
Target Names
smu-1
Alternative Names
smu-1 antibody; CC4.3Smu-1 suppressor of mec-8 and unc-52 protein antibody; Smu1 antibody; Suppressor of Mec and Unc defects 1 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans smu-1 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in pre-mRNA splicing as a component of the spliceosome. Selectively regulates alternative splicing of unc-52 exon 17. Thus, smu-1 mutants selectively suppress the effects of unc-52 nonsense mutations in exon 17 by promoting the accumulation of unc-52 isoforms that lack exon 17 and enhance the effects of unc-52 mutations that affect the exon 16 splice donor site. In contrast, smu-1 mutants do not suppress unc-52 nonsense mutations in exon 18.
Gene References into Functions
  1. SMU-2 and SMU-1 function together to regulate splice site choice in the pre-mRNAs of unc-52 and other genes PMID: 15254247
  2. Data show that loss-of-function mutations in the nonessential splicing factor smu-2 and smu-1 suppress unc-73 e936 uncoordination through changes in splicing. PMID: 19380478
Subcellular Location
Nucleus.
Protein Families
WD repeat SMU1 family
Tissue Specificity
Ubiquitous. Detected in the intestine and germline in adult hermaphrodites.
Database Links

KEGG: cel:CELE_CC4.3

STRING: 6239.CC4.3

UniGene: Cel.19360

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