Smurf Antibody

Code CSB-PA895578XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) Smurf Polyclonal antibody
Uniprot No.
Target Names
Smurf
Alternative Names
Smurf antibody; lack antibody; Smurf1 antibody; CG4943 antibody; E3 ubiquitin-protein ligase Smurf1 antibody; EC 2.3.2.26 antibody; HECT-type E3 ubiquitin transferase Smurf1 antibody; Lethal with a checkpoint kinase protein antibody; SMAD ubiquitination regulatory factor 1 homolog antibody; DSmurf antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) Smurf protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Down-regulates Dpp signaling after gastrulation by promoting MAD ubiquitination and subsequent degradation.
Gene References into Functions
  1. dSmurf is required for synapse remodeling and axon pruning by glia PMID: 28057437
  2. Interaction with Slimb leads to the ubiquitination and degradation of dSmurf, resulting in negative regulation of dSmurf-mediated Yki phosphorylation and activity in the Hippo pathway. Thus our study revealed a new regulatory mechanism of the Hippo pathway which may provide implications for developing tumor treatment. PMID: 27856247
  3. Sufficient knock-down of dSmurf increases the protein abundance of Wts and thus increases phosphorylation level at S168 of Yki, the key downstream target of Wts in the Hippo pathway. PMID: 25450375
  4. Smo interacts with Smurf and promotes it to mediate Patched ubiquitination by targeting the K1261 site in Ptc. PMID: 24302888
  5. Smurf-mediated differential proteolysis generates dynamic BMP signaling in germline stem cells during Drosophila testis development. PMID: 23988579

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Database Links

KEGG: dme:Dmel_CG4943

STRING: 7227.FBpp0305371

UniGene: Dm.2783

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