SNU114 Antibody

Code CSB-PA334069XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SNU114 Polyclonal antibody
Uniprot No.
Target Names
SNU114
Alternative Names
SNU114 antibody; GIN10 antibody; YKL173W antibody; YKL637Pre-mRNA-splicing factor SNU114 antibody; 114 kDa U5 small nuclear ribonucleoprotein component antibody; Growth inhibitory protein 10 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SNU114 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Component of the U5 snRNP complex required for pre-mRNA splicing. Binds GTP.
Gene References into Functions
  1. We propose that the U5 snRNA IL1 is a platform for protein binding and is required for , Brr2 and Snu114 association with the U5 snRNA to form the U5 snRNP. PMID: 23857713
  2. Data suggest that the RNA base pairing state is directly or indirectly sensed by the Snu114p G domain. PMID: 19620389
  3. GTP hydrolysis results in a rearrangement between Prp8 and the C terminus of Snu114 that leads to release of U1 and U4, thus activating the spliceosome for catalysis. PMID: 15911574
  4. Prp8 interaction site with Snu114 protein PMID: 16373487
  5. Assembly of Snu114 into U5 snRNP requires Prp8 and a functional GTPase domain. PMID: 16540695
  6. Snu114p serves as a signal-dependent switch that transduces signals to Brr2p to control spliceosome dynamics. PMID: 16885028
  7. Results report the structure of the Saccharomyces cerevisiae tri-snRNP, and show that U4/U6 snRNP forms a domain termed the arm, while a separate head domain adjacent to the arm harbors Brr2, and Prp8 and the GTPase Snu114 are located centrally. PMID: 18953335

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Subcellular Location
Nucleus.
Protein Families
TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, EF-G/EF-2 subfamily
Database Links

KEGG: sce:YKL173W

STRING: 4932.YKL173W

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