Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SNF3 Polyclonal antibody

Uniprot No.

P10870

Target Names

SNF3

Alternative Names

SNF3 antibody; YDL194W antibody; D1234 antibody; Low glucose sensor SNF3 antibody; High-affinity glucose receptor SNF3 antibody; High-affinity transporter-like sensor SNF3 antibody; Sucrose nonfermenting protein 3 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SNF3 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

High-affinity low glucose sensor that is part of the sensor/receptor-repressor (SSR) glucose-signaling pathway, which detects extracellular glucose and induces expression of glucose transporters that bring glucose into the cell. The transporter-like sensor generates an intracellular signal in the presence of low levels of glucose to promote low glucose-induced expression of HXT2. Binding of glucose to the SNF3 transmembrane domain activates a downstream signaling cascade, leading to phosphorylation of the RGT1 corepressors MTH1 and STD1, targeting them for SCF(Grr1)-dependent ubiquitination and degradation. Depletion of the corepressors robs RGT1 of its ability to repress expression of HXT genes, leading to accumulation of glucose transporters in the plasma membrane. SNF3 is involved as well in the transport of mannose and fructose. Even though SNF3 is similar to glucose transporters, it appears to be unable to transport glucose.

Gene References into Functions

Rgt2 and Snf3 glucose sensors are primary targets of methylglyoxal, which causes degradation of the Rgt2 and Snf3. PMID: 26764094
Mth1 and Std1, which are downstream effectors of the Snf3/Rgt2 pathway, were required for viability through mitochondrial function but not fermentative metabolism PMID: 25541485
Snf3 apparent affinity for glucose was lowest when the intracellular glucose concentration was high. The results conform to an alternating-access model for transporter-like sensors PMID: 20564191
This study reveals that Snf3, in addition to glucose, also senses fructose and mannose, as well as the glucose analogues 2-deoxyglucose, 3-O-methylglucoside and 6-deoxyglucose. PMID: 20014043
Suppression of the snf3 mutant phenotype by deletion of IRA2 was confirmed. PMID: 17311585

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Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

Major facilitator superfamily, Sugar transporter (TC 2.A.1.1) family

Database Links

KEGG: sce:YDL194W

STRING: 4932.YDL194W

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SNF3 Antibody

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