CSD2 Antibody

Code CSB-PA526782XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CSD2 Polyclonal antibody
Uniprot No.
Target Names
CSD2
Alternative Names
CSD2 antibody; KD-SOD antibody; SODCP antibody; At2g28190 antibody; F24D13.2Superoxide dismutase [Cu-Zn] 2 antibody; chloroplastic antibody; EC 1.15.1.1 antibody; Copper/zinc superoxide dismutase 2 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana CSD2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Destroys radicals which are normally produced within the cells and which are toxic to biological systems. Mediates tolerance to stress, including photo-oxidative stress.
Gene References into Functions
  1. In a series of grafting experiments using spl7 mutant and wild-type (WT) plants, expression of Cu-responsive microRNAs and the resulting suppression of CSD1 and CSD2 mRNAs were observed in leaves only when the aerial part was from WT plants, in which a low level of SPL7 was transcribed also in the vascular tissues. PMID: 29635212
  2. downregulation of CSD2 by the heat-inducible miR398 is required for thermotolerance in Arabidopsis. PMID: 23733060
  3. Data indicate that in the MKK5-RNAi (RNA interference) lines, the induction of CSD1 and CSD2 as well as the activation of MKK5 activity were completely arrested. PMID: 23677921
  4. Data indicate that transgenic plants expressing miR398-resistant forms of CSD1, CSD2 and CCS under the control of their native promoters are more sensitive to heat stress. PMID: 23480361
  5. miR398 links expression of the three major chloroplast copper proteins, plastocyanin, CCS1 and Csd2, to copper availability. PMID: 22841720
  6. CSD2 expression is fine-tuned by miR398-directed mRNA cleavage. PMID: 16861386
  7. Results show that CSD1, but not CSD2, mRNA levels were negatively correlated with miR398 levels during ozone, salinity and biotic stress. PMID: 19148671

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Subcellular Location
Plastid, chloroplast.
Protein Families
Cu-Zn superoxide dismutase family
Tissue Specificity
Expressed in leaves (at protein level). The spatial localization is regulated by miR398-mediated silencing. Mostly present in flowers, old rosette leaves and inflorescence, and, to a lower extent, in cauline leaves, stems and roots.
Database Links

KEGG: ath:AT2G28190

STRING: 3702.AT2G28190.1

UniGene: At.20409

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