Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SPP382 Polyclonal antibody

Uniprot No.

Q06411

Target Names

SPP382

Alternative Names

SPP382 antibody; CCF8 antibody; NTR1 antibody; YLR424W antibody; Pre-mRNA-splicing factor SPP382 antibody; CLF1 complex factor 8 antibody; Nineteen complex-related protein 1 antibody; NTC-related protein 1 antibody; Suppressor of PRP38 protein 2 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SPP382 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Involved in pre-mRNA splicing and spliceosome disassembly. Promotes release of excised lariat intron from the spliceosome by acting as a receptor for PRP43. This targeting of PRP43 leads to disassembly of the spliceosome with the separation of the U2, U5, U6 snRNPs and the NTC complex.

Gene References into Functions

Ntr1 and Ntr2 may act as 'doorkeepers' and both cofactors may inspect the RNP structure of spliceosomal complexes thereby targeting suboptimal spliceosomes for Prp43-mediated disassembly. PMID: 27923990
Prp43 associates with Ntr1 and Ntr2 to form the NTR complex which mediates the disassembly of spliceosome intermediates. PMID: 23166295
Spp382p interacts with multiple yeast splicing factors, including possible regulators of Prp43 DExD/H-Box protein function. PMID: 19581443
Ntr1-Ntr2-Prp43 complex catalyze disassembly of the spliceosome in an ATP-dependent manner PMID: 16357217
Ntr1 promotes release of excised introns from splicing complexes by acting as a spliceosome receptor or RNA-targeting factor for Prp43, possibly assisted by the Ntr2 protein. PMID: 16880513
Ntr1p interacts with Lif1p in a two-hybrid screen. PMID: 17389648
Ntr1 activates the Prp43 helicase to trigger release of lariat-intron from the spliceosome. PMID: 17875666
These results demonstrate that dynamic interactions of NTR with U5, through the interaction of Ntr2 with Brr2, and interactions of Ntr1 and Prp43 govern the recruitment of Prp43 to the spliceosome to mediate spliceosome disassembly. PMID: 17893323

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Subcellular Location

Cytoplasm. Nucleus.

Database Links

KEGG: sce:YLR424W

STRING: 4932.YLR424W

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SPP382 Antibody

Product Details

Customer Reviews and Q&A

Target Background