SPA2 Antibody

Datasheet

Code	CSB-PA326382XA01SVG
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Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SPA2 Polyclonal antibody

Uniprot No.

P23201

Target Names

SPA2

Alternative Names

SPA2 antibody; PEA1 antibody; YLL021W antibody; L1209Protein SPA2 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SPA2 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Involved in pheromone-induced morphogenesis and efficient mating, perhaps as a cytoskeletal protein.

Gene References into Functions

data indicate that Spa2 is recruited to the cleavage site to co-operate with the secretory vesicle system and particular actomyosin ring components to promote the incorporation of Chs2 into the so-called 'ingression progression complexes' during cytokinesis in budding yeast PMID: 29601579
the pea2-M409L mutation results in decreased invasive filamentation and elongation, diminished activity of a Kss1p MAPK pathway reporter, decreased unipolar budding, and diminished binding of the polarisome protein Spa2p. PMID: 25144783
Spa2p is involved in cytokinesis and cell wall morphogenesis. PMID: 16030260
Therefore, we suggest that Mid1 function is influenced by Spa2 function through polarized morphogenesis. PMID: 16087740
findings show Cdc42 serves an essential function in the establishment of bud site by catalyzing translocation of bud-site components such as Spa2 to the cell cortex; role of Cdc42 in the cortical localization of Spa2 appears to be independent of Cdc24 PMID: 16325503

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Subcellular Location

Cell tip. Note=Localizes to a sharp patch at the shmoo tip (mating projection) which corresponds to the site of polarized cell growth.

Database Links

KEGG: sce:YLL021W

STRING: 4932.YLL021W

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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SPA2 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background