Rz Antibody

Code CSB-PA360436XA01FSF
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Product Details

Full Product Name
Rabbit anti-Escherichia phage lambda (Bacteriophage lambda) Rz Polyclonal antibody
Uniprot No.
Target Names
Rz
Alternative Names
RzSpanin antibody; inner membrane subunit antibody; i-spanin antibody; Lysis protein Rz antibody
Raised in
Rabbit
Species Reactivity
Escherichia phage lambda (Bacteriophage lambda)
Immunogen
Recombinant Escherichia phage lambda (Bacteriophage lambda) Rz protein
Immunogen Species
Escherichia phage lambda (Bacteriophage lambda)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Component of the spanin complex that disrupts the host outer membrane and participates in cell lysis during virus exit. The spanin complex conducts the final step in host lysis by disrupting the outer membrane after holin and endolysin have permeabilized the inner membrane and degraded the host peptidoglycans. Host outer membrane disruption is due to local fusion between the inner and outer membrane performed by the spanin complex.
Gene References into Functions
  1. When coding sequences are aligned respective to the embedded genetic architecture of Rz1 within Rz, genetically silent domains of Rz1 correspond to mutationally sensitive domains in Rz, and vice versa, suggesting that the modular structure of the two subunits facilitated the evolutionary compression that resulted in the unique embedded gene architecture. PMID: 28040784
  2. Suppressors of non-functional Rz and Rz1 mutations largely encoded polar insertions within the hydrophobic core of the coiled-coil interface. PMID: 28468876
  3. A model is proposed in which the spanins carry out the essential step of disrupting the outer membrane, in a manner regulated by the state of the peptidoglycan layer. PMID: 22904283
  4. Here the authors present evidence that an Rz-Rz1 complex that spans the periplasm carries out the final step in the process of host lysis. PMID: 18713319
Subcellular Location
Host cell inner membrane; Single-pass type II membrane protein; Periplasmic side.
Protein Families
Caudovirales i-spanin family
Database Links

KEGG: vg:2703481

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