spen Antibody

Code CSB-PA854742XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) spen Polyclonal antibody
Uniprot No.
Target Names
spen
Alternative Names
spen antibody; CG18497 antibody; Protein split ends antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) spen protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Probable corepressor protein, which regulates different key pathways such as the EGF receptor and Wg pathways. Involved in neuronal cell fate, survival and axon guidance, cell cycle regulation and repression of head identity in the embryonic trunk. May act with the Hox gene Deformed and the EGF receptor signaling pathway. Positive regulator of the Wg pathway in larval tissues but not in embryonic tissues. May act as a transcriptional corepressor protein, which repress transcription via the recruitment of large complexes containing histone deacetylase proteins.
Gene References into Functions
  1. Spen is an important survival factor that ensures spatial control of the apoptotic wave that is necessary for the correct patterning and formation of the Drosophila eye. PMID: 25872184
  2. Split ends is a tissue/promoter specific regulator of Wingless signaling PMID: 12783785
  3. spen function is required for the correct formation and positioning of veins and mechanosensory bristles both on the anterior wing margin and on the notum, and for the maintenance of planar polarity PMID: 15596016
  4. Dosage-sensitive genetic interactions further support a model of functional antagonism between NITO and SPEN during Drosophila eye development. PMID: 16547102
  5. Plays a role in modulating output from the Notch and epidermal growth factor receptor pathways to ensure appropriate patterning during eye development. PMID: 17588724

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Subcellular Location
Nucleus.
Protein Families
RRM Spen family
Tissue Specificity
Ubiquitous. Expressed prior to cellularization in stage 3 embryos, and in blastoderm cells, including pole cells. Expressed throughout the rest of embryogenesis. Later, it is expressed at higher level in epidermal cells and CNS.
Database Links

KEGG: dme:Dmel_CG18497

STRING: 7227.FBpp0077781

UniGene: Dm.3450

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