spin Antibody

Code CSB-PA884411XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) spin Polyclonal antibody
Uniprot No.
Target Names
spin
Alternative Names
spin antibody; bnch antibody; CG8428 antibody; Protein spinster antibody; Protein benchwarmer antibody; Protein diphthong antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) spin protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Probable sphingolipid transporter that plays a central role in endosomes and/or lysosomes storage. Involved in TGF-beta-mediated synaptic growth regulation both pre- and postsynaptically via its function in endosomal storage regulation. Also required during oogenesis by regulating yolk spheres storage.
Gene References into Functions
  1. Within the migrating perineurial glial cells, Spinster is required to downregulate Decapentaplegic signaling activity, which ceases migratory abilities. Also, Spinster affects the growth of the carpet cell, which indirectly modulates glial migration. PMID: 21562262
  2. Spinster (Spin) encodes a putative lysosomal efflux permease with the hallmarks of a sugar transporter. Spin mutants accumulate lysosomal carbohydrates and enlarged lysosomes. PMID: 21518918
  3. spin promotes cell death mechanisms that are regulated negatively by th and emc and positively by atg8a PMID: 20091795
  4. Zebrafish yolk-specific not really started (nrs) gene is a vertebrate homolog of the Drosophila spinster gene and is essential for embryogenesis. PMID: 11836794
  5. Spin is expressed in nerve and muscle and is required for normal synaptic growth. Spin is found in a late endosomal compartment;there is evidence for altered endosomal/lysosomal function in Spin-deficient flies. PMID: 12408844

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Subcellular Location
Late endosome membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein.
Protein Families
Major facilitator superfamily, Spinster (TC 2.A.1.49) family
Tissue Specificity
Enriched in brain (at protein level).
Database Links

KEGG: dme:Dmel_CG8428

STRING: 7227.FBpp0088549

UniGene: Dm.3446

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