SPK1 Antibody

Datasheet

Code	CSB-PA913817XA01DOA
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SPK1 Polyclonal antibody

Uniprot No.

Q8SAB7

Target Names

SPK1

Alternative Names

SPK1 antibody; At4g16340 antibody; dl4200c antibody; FCAALL.346 antibody; Guanine nucleotide exchange factor SPIKE 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana SPK1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Customer Reviews and Q&A

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Target Background

Function

Guanine nucleotide exchange factor (GEF) for Rho and Rac. GEF proteins activate small GTPases by exchanging bound GDP for free GTP. Controls actin polymerization via the two heteromeric complexes WAVE and actin-related protein (ARP) 2/3. Involved in cytoskeletal reorganization required for cell shape (e.g. trichome and cotyledon) control and tissue development. Promotes polarized growth and cell-cell adhesion in the leaf epidermis probably by promoting the formation of endoplasmic reticulum (ER) exit site (ERES) and/or trafficking between the ER and Golgi. Triggers ARAC3/ROP6 activation required for auxin-mediated inhibition of PIN2 internalization during gravitropic responses (, PubMed:22683260).

Gene References into Functions

Results suggest that during the late petal development stages, SPIKE1 (SPK1), encoding a guanine nucleotide exchange factor and Rho of Plants (ROP) GTPase proteins (ROP2, ROP4, and ROP6)-dependent signaling module influences anisotropic growth in the petal and defines the final organ shape. PMID: 27440754
we demonstrated that SPIKE1 and Rho of Plants (ROP) GTPases were required for the final shape formation of conical cells, as KTN1 does. Live-cell imaging showed that wild-type cells exhibited random orientation of cortical microtubule arrays at early developmental stages but displayed a well-ordered circumferential orientation of microtubule arrays at later stages. PMID: 28644898
Data show that SPIKE1 is a peripheral membrane protein that accumulates at, and promotes the formation of, a specialized domain of the endoplasmic reticulum (ER) termed the ER exit site (ERES). PMID: 21109438
The DOCK family protein SPIKE1 (SPK1) is a GEF, and that one function of SPK1 is to control actin polymerization via two heteromeric complexes termed WAVE and actin-related protein (ARP) 2/3. PMID: 18308939

Subcellular Location

Cytoplasm. Endoplasmic reticulum membrane; Peripheral membrane protein. Nucleus.

Protein Families

DOCK family

Tissue Specificity

Expressed ubiquitously, in roots and aerial organs.

Database Links

KEGG: ath:AT4G16340

STRING: 3702.AT4G16340.1

UniGene: At.24408

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Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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