SPL Antibody

Datasheet

Code	CSB-PA181350XA01DOA
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SPL Polyclonal antibody

Uniprot No.

O81836

Target Names

SPL

Alternative Names

SPL antibody; NZZ antibody; At4g27330 antibody; F27G19.11 antibody; Protein SPOROCYTELESS antibody; Protein NOZZLE antibody; Transcription factor SPL antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana SPL protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Customer Reviews and Q&A

■ Customer Reviews

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Target Background

Function

Transcriptional regulator of sporocyte development. Acts as an adapter-like transcriptional repressor recruiting TPL/TPR corepressors to inhibit TCP transcription factors. Required for nucellus and embryo sac development. Plays a central role in patterning both the proximal-distal and the adaxial-abaxial axes during ovule development. Involved in establishing the prospective chalaza of the ovule and in controlling the cell number and the length of the funiculus, and is required for the development of the integuments. Required, with BEL1, for cytokinin-induced PIN1 expression in ovules. Involved in controlling stamen identity. May also regulate the morphology of lateral organs by repressing auxin production.

Gene References into Functions

SPL recruits topless/topless-related (TPL/TPR) co-repressors to inhibit the Cincinnata (CIN)-like Teosinte branched1/cycloidea/PCF (TCP) transcription factors. PMID: 25378179
The transcription factors BEL1 and SPL/NZZ, previously described as key regulators of ovule development, are needed for the auxin and cytokinin signaling pathways for the correct patterning of the ovule. PMID: 22786869
results demonstrate that the miR156-regulated SPLs establish a direct link between developmental programming and trichome distribution PMID: 20622149
SPL/NZZ is not only essential for early anther cell differentiation but also is engaged in controlling stamen identity in Arabidopsis PMID: 19726570
Nozzle protein may coordinate proximal-distal and adaxial-abaxial polarity establishment in the developing ovule by interacting with YABBY proteins in vitro in Arabidopsis. [Nozzle (NZZ)] PMID: 15299139
The cell-free method was used to synthesize SPL as a correctly folded and functional zinc-binding protein. PMID: 17146616

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Subcellular Location

Nucleus.

Protein Families

NOZZLE family

Tissue Specificity

Expressed in flower buds. Not found in leaves, siliques and stems. Detected in rosette leaves, stem tissue and seedlings.

Database Links

KEGG: ath:AT4G27330

STRING: 3702.AT4G27330.1

UniGene: At.2342

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