Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SPL7 Polyclonal antibody

Uniprot No.

Q8S9G8

Target Names

SPL7

Alternative Names

SPL7 antibody; At5g18830 antibody; F17K4.80 antibody; Squamosa promoter-binding-like protein 7 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana SPL7 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Transcription factor that participates in reprogramming global gene expression during copper deficiency in order to improve the metal uptake and prioritize its distribution to copper proteins of major importance (Probable). Binds directly to 5'-GTAC-3' motifs in the microRNA (miRNA) promoter of the stress-responsive miRNAs miR398b and miR398c to activate their transcription. During copper deficiency, activates the copper transporters COPT1 and COPT2, and the copper chaperone CCH, directly or indirectly via miRNAs. Required for the expression of the miRNAs miR397, miR408 and miR857. Acts coordinately with HY5 to regulate miR408 and its target genes in response to changes in light and copper conditions. Activates miR857 and its target genes in response to low copper conditions. Involved in cadmium stress response by regulating miR397a, miR398b, miR398c and miR857. Required for iron homeostasis during copper deficiency.

Gene References into Functions

Cytological and biochemical approaches confirmed an operative transmembrane domain (TMD) and uncovered a dual localisation of SPL7 between the nucleus and an endomembrane system, most likely the endoplasmic reticulum (ER). PMID: 25207797
The data presented here provide evidence for SPL7 and KIN17 protein interaction as a point of convergence in response to both Copper deficiency and oxidative stress. PMID: 24335506
SPL7 directly regulates miR408 expression. PMID: 23289771
COPT6 expression is regulated by copper availability that, in part, is controlled by a master regulator of copper homeostasis, SPL7 PMID: 22865877
Cu uptake into roots of Cu-deficient plants is dependent on FRO4/FRO5 and SPL7. PMID: 22374396
SPL7 activates the transcription of multiple genes involved in copper homeostasis. PMID: 19122104

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Subcellular Location

Nucleus speckle.

Tissue Specificity

Expressed in roots rosette leaves, cauline leaves, stems, flowers and siliques.

Database Links

KEGG: ath:AT5G18830

STRING: 3702.AT5G18830.3

UniGene: At.495

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SPL7 Antibody

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