Product Details

Full Product Name

Rabbit anti-Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) spm1 Polyclonal antibody

Uniprot No.

Q92398

Target Names

spm1

Alternative Names

spm1 antibody; pmk1 antibody; SPBC119.08 antibody; Mitogen-activated protein kinase spm1 antibody; MAP kinase spm1 antibody; EC 2.7.11.24 antibody; MAP kinase pmk1 antibody

Raised in

Rabbit

Species Reactivity

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Immunogen

Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) spm1 protein

Immunogen Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Regulates cell integrity and functions coordinately with the protein kinase C pathway (pck1 and pck2). Involved the regulation of wall architecture, cell shape, cytokinesis in exponential and stationary phase, and metabolism of ions.

Gene References into Functions

Threonine-monophosphorylated Pmk1 was able to execute most of the biological functions of the dually phosphorylated kinase. PMID: 26432170
T842 phosphorylation is not essential for Pmk1 activation, and putative phosphorylation at T846 might play an additional role in Pck2 catalytic activation and downstream signaling PMID: 25416816
Consistently, the phosphorylation of Pmk1 MAPK on heat shock was decreased in the cwg2-v2 mutants, and rho4- and rho5-null cells. PMID: 25651781
Data indicate that mutation of any of the two first amino acids in the IYT motif led to a failure of inactive dual-specificity MAPK phosphatases Sdp1 to trap mitogen-activated protein kinase Slt2. PMID: 24465549
Results suggest that Activation of Atf1 is not fully dependent on Sty1 and Pmk1. PMID: 22661707
down-regulation of nuclear Pmk1 by MAPK phosphatases induced by the stress activated protein kinase pathway is important for the fine modulation of extranuclear Pmk1 activity. PMID: 22685296
Results suggest that Ecm33 gene expression is regulated by Atf1 and Mbx1, and taht Ecm33 is involved in the negative feedback regulation of Pmk1 cell integrity signaling and is linked to cellular Ca(2+) signaling. PMID: 20032302
analysis of the Pmk1 cell integrity pathway in Schizosaccharomyces pombe PMID: 16291757
These results demonstrate that Cpp1 is a key component of the Pck2-Pmk1 signaling through the spatial control of the small GTPase Rho2. PMID: 17005909
the stress-activated protein kinase (SAPK) pathway and its main effector, Sty1p MAPK, are essential for proper deactivation of Pmk1p under hypertonic stress in a process regulated by Atf1p transcription factor PMID: 17761528
Atf1 is a key component of the cell integrity signaling downstream of Pmk1 MAPK PMID: 17881729
Pmk1 mitogen-activated protein kinase (MAPK) directly phosphorylates Nrd1, thereby negatively regulating the binding activity of Nrd1 to Cdc4 mRNA. PMID: 19279143

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Protein Families

Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily

Database Links

KEGG: spo:SPBC119.08

STRING: 4896.SPBC119.08.1

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spm1 Antibody

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