spn-F Antibody

Code CSB-PA136468XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) spn-F Polyclonal antibody
Uniprot No.
Target Names
spn-F
Alternative Names
spn-F antibody; CG12114 antibody; Protein spindle-F antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) spn-F protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Plays a role in oocyte axis determination and microtubule organization during oogenesis. Also required for polarized organization of the bristle. Required, with jvl, for activation of the kinase IKKepsilon in the germ line. Also required for localization of IKKepsilon to the distal tip of elongating bristles by acting as an adapter linking IKKepsilon and cytoplasmic dynein. Involved in dendrite pruning in larval sensory neurons during metamorphosis.
Gene References into Functions
  1. IkappaB kinase 2 binds physically to Spindle-F protein, biomolecular interaction analysis of Spindle-F protein and IkappaB kinase 2 demonstrating that both proteins bind directly and form a complex PMID: 18796167
Subcellular Location
Cytoplasm, cytoskeleton. Cytoplasm. Cell projection, axon. Cell projection, dendrite. Perikaryon.
Tissue Specificity
In pupal bristles, localizes to the bristle tip throughout the elongation period (at protein level).
Database Links

KEGG: dme:Dmel_CG12114

UniGene: Dm.1384

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