Product Details

Full Product Name

Rabbit anti-Mus musculus (Mouse) Spo11 Polyclonal antibody

Uniprot No.

Q9WTK8

Target Names

Spo11

Alternative Names

Spo11Meiotic recombination protein SPO11 antibody; EC 5.6.2.2 antibody

Raised in

Rabbit

Species Reactivity

Mus musculus (Mouse)

Immunogen

Recombinant Mus musculus (Mouse) Spo11 protein

Immunogen Species

Mus musculus (Mouse)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Component of a topoisomerase 6 complex specifically required for meiotic recombination. Together with TOP6BL, mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination. The complex promotes relaxation of negative and positive supercoiled DNA and DNA decatenation through cleavage and ligation cycles. Essential for the phosphorylation of SMC3, HORMAD1 and HORMAD2.; In contrast to isoform 1, does not mediate DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination.

Gene References into Functions

These data suggest that Tex19.1 and Ubr2 are required for mouse spermatocytes to accumulate sufficient Spo11-dependent recombination to ensure that the homology search is consistently successful, and reveal a hitherto unknown genetic pathway promoting meiotic recombination in mammals. PMID: 28708824
If SPO11 expression is reduced and DNA double-strand breaks at zygonema are decreased (approximately 40 % below wild-type level), meiotic chromosome pairing is normal. PMID: 26440409
suppression of genetic recombination in the pseudoautosomal region and at subtelomeres in mice with a hypomorphic Spo11 allele PMID: 23870400
our results indicate that in addition to asynapsis, persistent SPO11-induced DSBs are important for the initiation of MSCI and MSUC, and that SPO11-independent DNA repair foci contribute to the MSUC response in oocytes. PMID: 23754961
Mice with greater or fewer copies of the Spo11 gene--with correspondingly greater or fewer numbers of early recombination foci--exhibited relatively invariant crossover numbers. PMID: 22388890
Data support a role for SPO11alpha in mid- to late prophase, presumably acting as a topoisomerase, that would be conserved in male and female meiocytes. PMID: 20647542
required for meiotic chromosome synapsis PMID: 11106738
chromosome synapsis defects and sexually dimorphic meiotic progression in Spo11 deficient mice PMID: 11106739
Spo11 is not required for Ig hypermutation or class switch recombination PMID: 11807770
In Spo11 -/- cells, SMC1 localization on meiotic chromosomes is mainly associated with homologous or non-homologous synapsed regions, whereas SMC3 localized throughout the chromosomes. Both localized along chromosome cores of Spo11 null spermatocytes. PMID: 12498344
meiotic double-stranded breaks are processed by endonucleolytic cleavage that releases Spo11 attached to an oligonucleotide with a free 3'-OH PMID: 16107854
The presence of these L-foci in Spo11(-/-) spermatocytes raises the interesting possibility of yet uncharacterized alterations in DNA or chromosome structure in Spo11(-/-) cells. PMID: 17456548
Detection of the release product, SPO11-oligonucleotide complexes, from mouse testis lysates. PMID: 19799184

Hide All

Subcellular Location

Nucleus.

Protein Families

TOP6A family

Tissue Specificity

High levels are found only in the testis where expression is restricted primarily to meiotic germ cells. Not expressed in spermatogonia. Highest levels are found in pachytene spermatocytes. Very low levels are found in thymus, brain and oocytes of embryon

Database Links

KEGG: mmu:26972

STRING: 10090.ENSMUSP00000059056

UniGene: Mm.23495

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Spo11 Antibody

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