Spt5 Antibody

Code CSB-PA895565XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) Spt5 Polyclonal antibody
Uniprot No.
Target Names
Spt5
Alternative Names
Spt5 antibody; CG7626 antibody; Transcription elongation factor SPT5 antibody; DRB sensitivity-inducing factor large subunit antibody; DSIF large subunit antibody; dSpt5 antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) Spt5 protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates transcription elongation by RNA polymerase II. DSIF enhances transcriptional pausing at sites proximal to the promoter, which may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF may also promote transcriptional elongation within coding regions. DSIF is required for the transcriptional induction of heat shock response genes and regulation of genes which control anterior-posterior patterning during embryonic development.
Gene References into Functions
  1. KOW5 is involved in promoter-proximal pausing through contact with the nascent RNA PMID: 28213523
  2. Spt5 acts both positively and negatively on transcription in vivo during heat shock induction. PMID: 15380072
Subcellular Location
Nucleus. Chromosome. Note=Localizes predominantly to transcriptionally active regions of polytene chromosomes.
Protein Families
SPT5 family
Database Links

KEGG: dme:Dmel_CG7626

STRING: 7227.FBpp0085716

UniGene: Dm.2263

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