Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SPY Polyclonal antibody

Uniprot No.

Q96301

Target Names

SPY

Alternative Names

SPY antibody; At3g11540 antibody; F24K9.29 antibody; Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY antibody; EC 2.4.1.255 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana SPY protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Probable O-linked N-acetylglucosamine transferase (OGT) involved in various processes such as gibberellin (GA) signaling pathway and circadian clock. OGTs catalyze the addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Probably acts by adding O-linked sugars to yet unknown proteins. Acts as a repressor of GA signaling pathway to inhibit hypocotyl elongation. Functions with GIGANTEA (GI) in pathways controlling flowering, circadian cotyledon movements and hypocotyl elongation. Acts as a light-regulated promoter of elongation via its interaction with GI. Acts as an activator of cytokinin signaling. Required with SEC for gamete and seed development. Its OGT activity has been proved in vitro but not in vivo.

Gene References into Functions

posttranslational modification of TCP14/15 by SPY inhibits their proteolysis. PMID: 27208284
The study revealed that SPY-dependent protein O-fucosylation plays a key role in regulating plant development. PMID: 28244988
SPINDLY regulates cortex proliferation by maintaining cellular redox homeostasis PMID: 25267734
SPY plays a negative role in plant abiotic stress tolerance, probably by integrating environmental stress signals via gibberellic acid and Cytokinins cross talk. PMID: 22013217
Data suggest that responses of leaves and flowers to CK are mediated by SPY-dependent TCP14 and TCP15 activities. PMID: 22267487
spy-4 partially suppressed the reduced abundance of CONSTANS and FLOWERING LOCUS T RNA and the late flowering of gigantea mutant plants PMID: 15155885
Our results demonstrate that SPY acts as both a repressor of GA responses and a positive regulator of cytokinin signaling. Hence, SPY may play a central role in the regulation of GA/cytokinin cross talk during plant development. PMID: 15608330
SPY acts in the cytosol to suppress gibberellin responses and to promote cytokinin signaling. PMID: 19228341

Hide All

Subcellular Location

Cytoplasm. Nucleus.

Protein Families

Glycosyltransferase 41 family, O-GlcNAc transferase subfamily

Tissue Specificity

Widely expressed. Present throughout the plant (at protein level).

Database Links

KEGG: ath:AT3G11540

STRING: 3702.AT3G11540.1

UniGene: At.17656

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SPY Antibody

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