Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SR45 Polyclonal antibody

Uniprot No.

Q9SEE9

Target Names

SR45

Alternative Names

SR45 antibody; RNPS1 antibody; At1g16610 antibody; F19K19.9 antibody; Serine/arginine-rich splicing factor SR45 antibody; At-SR45 antibody; AtSR45 antibody; Serine/arginine-rich ribonucleoprotein 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana SR45 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Involved in 5' and 3' splicing site selection of introns, and may bridge the 5' and 3' components of the spliceosome. Isoform 1 is required during flower petal development and isoform 2 is involved in root growth. Regulates negatively glucose and abscisic acid (ABA) signaling during early seedling development. Involved in the RNA-directed DNA methylation pathway. Modulates KIN10 stability in response to sugars, probably through the splicing regulation of 5PTASE13, a protein implicated in the proteasomal degradation of KIN10.

Gene References into Functions

SR45 plays an unexpected role in mRNA processing of intronless genes, and numerous abscisic acid (ABA) signaling genes are targeted for regulation at the posttranscriptional level. PMID: 26603559
SR45 is a negative regulator of sugar signaling during early seedling development and is involved in the repression of glucose-induced ABA accumulation and down-regulation of ABA biosynthesis PMID: 20699397
Data show that the mobility of SR45 and SR1/SRp34 is dependent on ATP, phosphorylation and transcription. PMID: 16895966
The generation of 6 variants of SR45 and their molecular characterization are reported. PMID: 17556373
atSR45a proteins help to form the bridge between 5' and 3' splice sites in the spliceosome assembly. PMID: 19238562
The two alternatively spliced isoforms of SR$% have distinct biological functions. PMID: 19403727

Hide All

Subcellular Location

Nucleus speckle. Nucleus, nucleoplasm.

Protein Families

Splicing factor SR family, SR45 subfamily

Tissue Specificity

Especially present in actively growing regions and dividing cells. Mostly expressed in roots (primary and secondary root meristem), shoot apical meristem (SAM), leaf primordia, pollen and inflorescence, and, to a lower extent, in leaves, vascular tissue,

Database Links

KEGG: ath:AT1G16610

STRING: 3702.AT1G16610.3

UniGene: At.11865

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SR45 Antibody

Product Details

Customer Reviews and Q&A

Target Background