Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) Src42A Polyclonal antibody

Uniprot No.

Q9V9J3

Target Names

Src42A

Alternative Names

Src42A antibody; Src41 antibody; TK5 antibody; CG44128 antibody; Tyrosine-protein kinase Src42A antibody; EC 2.7.10.2 antibody; Tyrosine-protein kinase Src41 antibody; Dsrc41 antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) Src42A protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Required directly or indirectly for the phosphorylation of drpr which is necessary for the interaction of drpr with shark and subsequent glial phagocytic activity. Together with drpr and shark, promotes the migration of macrophages to sites of wounding as part of a signaling cascade where Scr42a detects production of hydrogen peroxide at wound sites which triggers phosphorylation of drpr and subsequent recruitment and activation of shark. Essential for correct eye morphogenesis (ommatidial R7 neuron formation) which requires the Ras1/MAPK signal transduction pathway. May be involved in the regulation of cytoskeleton organization and cell-cell contacts in developing ommatidia. During embryogenesis, involved in regulation of dorsal closure where it may have a role in activating the JNK pathway in leading edge cells during this process.

Gene References into Functions

Src overexpression alone activated the Jun N-terminal Kinase (JNK) signalling pathway to promote actin cytoskeletal and cell polarity defects and drive apoptosis in in a whole epithelial tissue context in the Drosophila eye, whereas, in cooperation with RasACT, JNK led to a loss of differentiation and an invasive phenotype. PMID: 29861494
show the same signaling occurs in Drosophila inflammatory cells in response to wound-induced H2O2 with mutants for the Lyn homolog Src42A displaying impaired inflammatory migration to wounds PMID: 26028435
Reduction of Src42A function leads to nuclear translocation and activation of FOXO proteins. PMID: 24513978
Results show that Src42a and Src64b are required for the normal division capacity of Drosophila intestinal progenitor cells, and that these genes as well as Ack cause progenitor overproliferation in the intestine via core cell cycle activation. PMID: 24975577
The tyrosine kinases Brk/PTK6/Sik, Srm, Frk/Rak/Gtk/Iyk/Bsk, and Src42A/Dsrc41 have a low degree of sequence homology to other known kinases. The exon structure of these kinases, called the Brk family, is highly conserved and distinct. PMID: 12725532
Src42A is required (with Src64) for Armadillo tyrosine residue phosphorylation, and is essential for proper cell-cell matching during dorsal closure. PMID: 15857910
Src42a is required for tracheal development and to increase the rate of adherens junction turnover. PMID: 18305002
Draper-Src42A-Shark interactions are strikingly similar to mammalian immunoreceptor-SFK-Syk signalling events in mammalian myeloid and lymphoid cells. PMID: 18432193
Genetic analyses imply that FAK mediates the signaling pathway of the alphaPS3 integrin and beta[nu] integrin heterodimer and functions redundantly with Src42A. PMID: 18925939

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Protein Families

Protein kinase superfamily, Tyr protein kinase family, SRC subfamily

Tissue Specificity

Ubiquitous in early embryos, in stages 13-16 expression is seen in visceral mesoderm, hindgut, brain, anal pads and ventral ganglions. In larvae, expression is in CNS, wing disk, leg disk and photoreceptor precursors in the eye-antenna disks posterior to

Database Links

KEGG: dme:Dmel_CG44128

STRING: 7227.FBpp0085320

UniGene: Dm.4134

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Src42A Antibody

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