SRS2 Antibody

1. Two main activities of Srs2, strippase and helicase, counteract toxic joint intermediates formed during break-induced replication by preventing their formation and promoting their disruption, respectively. PMID: 29176630
2. Srs2 rapidly disrupts small heteroduplex DNA joints bound to Rad51 filaments. PMID: 29241544
3. These data establish a biochemical mechanism for the role of Srs2 in crossover suppression by promoting synthesis-dependent strand annealing through disruption of DNA polymerase delta-extended D-loops. PMID: 28535142
4. Uls1 contributes to the pathway where intermediates generated at replication forks are dismantled by Srs2 bound to SUMO-PCNA. Upon ULS1 deletion, accumulating Srs2-SUMO-unable to bind PCNA-takes part in an alternative PCNA-independent recombination repair salvage pathway(s) PMID: 28341648
5. Srs2 unfolds trinucleotide repeat hairpin repetitively, and its activity depends on the folding strength and the total length of TNR hairpin. PMID: 26004439
6. When Rad51 is bound on the double-stranded DNA, its interaction with Srs2 blocks the helicase (DNA unwinding) activity of Srs2. PMID: 24576606
7. Srs2 exhibits ATP-dependent repetitive motion on single-stranded DNA and this activity prevents re-formation of the Rad51 filament. PMID: 23939144
8. Rad52 mutant cannot restore viability of srs2Delta cells that accumulate intertwined recombination intermediates which are normally processed by Srs2 post-synaptic functions PMID: 24130504
9. Srs2 disrupts Rad51-containing complexes from chromosomes during meiosis. PMID: 23770697
10. Putative antirecombinase Srs2 DNA helicase promotes noncrossover homologous recombination avoiding loss of heterozygosity. PMID: 24043837
11. The hDNA data are consistent with the proposed role of Sgs1 in the dissolution of double HJs, and we propose that Srs2 dismantles nicked HJs. PMID: 23516370
12. Srs2 unwinding is inhibited by ssDNA binding protein RPA in vitro PMID: 22921573
13. The SUMO-interacting motif region is required for Srs2 sumoylation, likely by binding to SUMO-charged Ubc9. PMID: 22705796
14. suppression of homologous recombination in postreplication repair-deficient cells by Srs2 and PCNA sumoylation is required for checkpoint activation and checkpoint maintenance during UV irradiation PMID: 20713444
15. the importance of the ATP hydrolysisfueled DNA motor activity in SRS2 functions PMID: 15047689
16. suppression of homeologous recombination not relaxed in the srs2 mutant PMID: 15611162
17. Srs2 assoiates with Mre11 in different subcomplexes following checkpoint activation and CDK1-mediated Srs2 phosphorylation. PMID: 15964827
18. These findings suggest that the hFBH1 helicase is a functional human orthologue of budding yeast Srs2 that also possesses self-regulation properties necessary to execute its recombination functions. PMID: 17724085
19. The Srs2 helicase activity is stimulated by Rad51 filaments on dsDNA PMID: 18243118
20. Srs2 maintains its facile unwinding of triplet repeat hairpins embedded within duplex DNA, supporting the genetic evidence that Srs2 is a key helicase in Saccharomyces cerevisiae for preventing expansions. PMID: 18440969
21. Description of two new srs2 mutants that have acquired the ability to perform recombinational repair in a rad18 context, but have retained the ability to reverse toxic structures in a RAD18 context. PMID: 18658248
22. Results suggest that Srs2 promotes fork reversal in repetitive sequences, preventing repeat instability and fragility; in the absence of Srs2 or Sgs1, DNA damage accumulates and is processed by homologous recombination, triggering repeat rearrangements. PMID: 19136956
23. Results characterize Srs2 function in vivo and describe a novel mechanism for regulating the initiation of homologous recombination. PMID: 19506039
24. Data show that a physical interaction between Rad51 and the C-terminal region of Srs2 triggers ATP hydrolysis within the Rad51 filament, causing Rad51 to dissociate from DNA. PMID: 19595720
25. functional significance of the Rad51-Rad52 and Rad51-Srs2 complexes in the control of presynaptic filament assembly and disassembly. PMID: 19605344
26. Translocations rely on homologous recombination (HR) proteins, such as DNA repair enzymes, as well as on the break-induced replication-specific protein Pol32 and on Srs2 PMID: 19651902

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KEGG: sce:YJL092W

STRING: 4932.YJL092W