Product Details

Purity

>85% (SDS-PAGE)

Target Names

SRS2

Uniprot No.

P12954

Alternative Names

SRS2; HPR5; RADH; YJL092W; J0913ATP-dependent DNA helicase SRS2; EC 3.6.4.12

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

ATP-dependent DNA helicase involved in DNA repair at least for UV-induced lesions. The polarity of the helicase activity was determined to be 3' to 5'.

Gene References into Functions

Two main activities of Srs2, strippase and helicase, counteract toxic joint intermediates formed during break-induced replication by preventing their formation and promoting their disruption, respectively. PMID: 29176630
Srs2 rapidly disrupts small heteroduplex DNA joints bound to Rad51 filaments. PMID: 29241544
These data establish a biochemical mechanism for the role of Srs2 in crossover suppression by promoting synthesis-dependent strand annealing through disruption of DNA polymerase delta-extended D-loops. PMID: 28535142
Uls1 contributes to the pathway where intermediates generated at replication forks are dismantled by Srs2 bound to SUMO-PCNA. Upon ULS1 deletion, accumulating Srs2-SUMO-unable to bind PCNA-takes part in an alternative PCNA-independent recombination repair salvage pathway(s) PMID: 28341648
Srs2 unfolds trinucleotide repeat hairpin repetitively, and its activity depends on the folding strength and the total length of TNR hairpin. PMID: 26004439
When Rad51 is bound on the double-stranded DNA, its interaction with Srs2 blocks the helicase (DNA unwinding) activity of Srs2. PMID: 24576606
Srs2 exhibits ATP-dependent repetitive motion on single-stranded DNA and this activity prevents re-formation of the Rad51 filament. PMID: 23939144
Rad52 mutant cannot restore viability of srs2Delta cells that accumulate intertwined recombination intermediates which are normally processed by Srs2 post-synaptic functions PMID: 24130504
Srs2 disrupts Rad51-containing complexes from chromosomes during meiosis. PMID: 23770697
Putative antirecombinase Srs2 DNA helicase promotes noncrossover homologous recombination avoiding loss of heterozygosity. PMID: 24043837
The hDNA data are consistent with the proposed role of Sgs1 in the dissolution of double HJs, and we propose that Srs2 dismantles nicked HJs. PMID: 23516370
Srs2 unwinding is inhibited by ssDNA binding protein RPA in vitro PMID: 22921573
The SUMO-interacting motif region is required for Srs2 sumoylation, likely by binding to SUMO-charged Ubc9. PMID: 22705796
suppression of homologous recombination in postreplication repair-deficient cells by Srs2 and PCNA sumoylation is required for checkpoint activation and checkpoint maintenance during UV irradiation PMID: 20713444
the importance of the ATP hydrolysisfueled DNA motor activity in SRS2 functions PMID: 15047689
suppression of homeologous recombination not relaxed in the srs2 mutant PMID: 15611162
Srs2 assoiates with Mre11 in different subcomplexes following checkpoint activation and CDK1-mediated Srs2 phosphorylation. PMID: 15964827
These findings suggest that the hFBH1 helicase is a functional human orthologue of budding yeast Srs2 that also possesses self-regulation properties necessary to execute its recombination functions. PMID: 17724085
The Srs2 helicase activity is stimulated by Rad51 filaments on dsDNA PMID: 18243118
Srs2 maintains its facile unwinding of triplet repeat hairpins embedded within duplex DNA, supporting the genetic evidence that Srs2 is a key helicase in Saccharomyces cerevisiae for preventing expansions. PMID: 18440969
Description of two new srs2 mutants that have acquired the ability to perform recombinational repair in a rad18 context, but have retained the ability to reverse toxic structures in a RAD18 context. PMID: 18658248
Results suggest that Srs2 promotes fork reversal in repetitive sequences, preventing repeat instability and fragility; in the absence of Srs2 or Sgs1, DNA damage accumulates and is processed by homologous recombination, triggering repeat rearrangements. PMID: 19136956
Results characterize Srs2 function in vivo and describe a novel mechanism for regulating the initiation of homologous recombination. PMID: 19506039
Data show that a physical interaction between Rad51 and the C-terminal region of Srs2 triggers ATP hydrolysis within the Rad51 filament, causing Rad51 to dissociate from DNA. PMID: 19595720
functional significance of the Rad51-Rad52 and Rad51-Srs2 complexes in the control of presynaptic filament assembly and disassembly. PMID: 19605344
Translocations rely on homologous recombination (HR) proteins, such as DNA repair enzymes, as well as on the break-induced replication-specific protein Pol32 and on Srs2 PMID: 19651902

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Subcellular Location

Nucleus.

Protein Families

Helicase family, UvrD subfamily

Database Links

KEGG: sce:YJL092W

STRING: 4932.YJL092W

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Recombinant Saccharomyces cerevisiae ATP-dependent DNA helicase SRS2 (SRS2), partial

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