SSY1 Antibody

Code CSB-PA695758XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SSY1 Polyclonal antibody
Uniprot No.
Target Names
SSY1
Alternative Names
SSY1 antibody; APF7 antibody; SHR10 antibody; YDR160W antibody; YD8358.14 antibody; SPS-sensor component SSY1 antibody; Amino-acid permease homolog SSY1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SSY1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Amino acid sensor component of the SPS-sensor system, which regulates the expression of several amino acid-metabolizing enzymes and amino acid- and peptide-permeases in response to extracellular amino acid levels by controlling the activity of two transcription factors, STP1 and STP2. Amino-acid permease homolog that seems to interact directly with the extracellular signaling molecules, but has no amino acid transporter activity. May recruit casein kinases YCK1 and YCK2 to hyperphosphorylate and activate downstream component PTR3 in response to amino acid stimulus.
Gene References into Functions
  1. Ssy1 localization depends on the presence of a PM-binding domain, an intrinsically disordered linker region of sufficient length. PMID: 25409870
  2. SSY1, a tranporter-like sensor, allows the cell to respond to changes in nutrient availability through detection of the relative concentrations of intra- and extracellular ligand. PMID: 16651382
  3. SSY1 mutants that constitutively activate a target promoter, were isolated. PMID: 18307103
  4. Data confirm that a frameshift mutation in a mononucleotide run of eight consecutive T's in the coding region SSY1 causes dramatic differences in gene-expression levels of key genes involved in amino acid biosynthesis. PMID: 18422925
Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
Amino acid-polyamine-organocation (APC) superfamily
Database Links

KEGG: sce:YDR160W

STRING: 4932.YDR160W

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