SSZ1 Antibody

Code CSB-PA327890XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SSZ1 Polyclonal antibody
Uniprot No.
Target Names
SSZ1
Alternative Names
SSZ1 antibody; PDR13 antibody; YHR064C antibody; Ribosome-associated complex subunit SSZ1 antibody; DnaK-related protein SSZ1 antibody; Heat shock protein 70 homolog SSZ1 antibody; Pleiotropic drug resistance protein 13 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SSZ1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Component of the ribosome-associated complex (RAC), a heterodimeric chaperone complex involved in regulation of accurate translation termination and in folding or maintaining nascent polypeptides in a folding-competent state. RAC stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones SSB1/SSB2 that bind to the nascent polypeptide chain. SSZ1 is required for ZUO1 to function efficiently as a J-protein for SSB1/SSB2. Also involved in pleiotropic drug resistance by post-translational activation of transcription factor PDR1.
Gene References into Functions
  1. study presents structural analyses of the ribosome-associated complex (RAC) consisting (Hsp70) Ssz1 and the Hsp40 Zuo1; crystal structure of the Ssz1 ATPase domain bound to ATP-Mg(2) explains its catalytic inactivity and suggests Ssz1 may act before the RAC-associated chaperone Ssb PMID: 23202586
  2. Ssz1 activates Pdr1 causing premature growth arrest of cells during the diauxic shift, through quorum sensing, as they adapt to the changing environmental conditions. PMID: 22203981
  3. Ssz1 binds ATP, but none of the 11 different amino acid substitutions in the ATP-binding cleft affected Ssz1 function in vivo. PMID: 15908962
Subcellular Location
Cytoplasm.
Protein Families
Heat shock protein 70 family
Database Links

KEGG: sce:YHR064C

STRING: 4932.YHR064C

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