stan Antibody

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Code	CSB-PA895571XA01DLU
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Target Background

Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) stan Polyclonal antibody

Uniprot No.

Q9V5N8

Target Names

stan

Alternative Names

stan antibody; fmi antibody; CG11895 antibody; Protocadherin-like wing polarity protein stan antibody; Protein flamingo antibody; Protein starry night antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) stan protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Involved in the fz signaling pathway that controls wing tissue polarity. Also mediates homophilic cell adhesion. May play a role in initiating prehair morphogenesis. May play a critical role in tissue polarity and in formation of normal dendrite fields. During planar cell polarity, stabilizes asymmetric PCP domains together with ATP6AP2.

Gene References into Functions

microRNA-9a regulates dendrite growth through cadherin protein Flamingo-G protein Galphaq signaling in Drosophila sensory neurons PMID: 26016469
Fmi and Gogo function downstream of Otd in retina. PMID: 26114289
The planar cell polarity gene prickle interacts with flamingo in regulating sensory axon advance at a key choice point - the transition between the peripheral nervous system and the central nervous system. PMID: 21827745
Espinas (Esn), binds to an intracellular juxtamembrane domain of Flamingo (Fmi), and that this Fmi-Esn interplay elicits repulsion between dendritic branches of Drosophila sensory neurons PMID: 21937715
Data suggest that Gogo acts as a functional partner of Fmi for R8 photoreceptor axon targeting and that the dynamic regulation of their interaction specifies synaptic-layer selection of photoreceptors. PMID: 21317905
The atypical cadherin Flamingo (Fmi) plays critical, but distinct, roles in distinct fate specification of photoreceptors R3 and R4. PMID: 12015972
Flamingo plays a role in early neuronal differentiation and exerts specific effects on dendrites and axons PMID: 12074553
Autonomously required for the establishment of ommatidial polarity. PMID: 12642492
Flamingo occurs in retinal growth cones. Loss of Flamingo function in retinal cells disrupts the local pattern of synaptic terminals in the medulla; Flamingo is transiently expressed in retinal axons as they enter the target region. PMID: 12754514
Flamingo expresses two differentially spliced RNAs in wing imaginal discs, which encode two isoforms of the atypical cadherin Flamingo. PMID: 17304516
Atypical cadherin Flamingo functions as a short-range, homophilic signal, passing between specific R cell growth cones to influence postsynaptic targeting. PMID: 18400160
Study provides evidence that Fmi functions instructively to mediate Fz-Vang intercellular signal relay, recruiting Fz and Vang to opposite sides of cell boundaries. PMID: 18555784
Flamingo protein binds preferentially to the Fz:Dsh complex and controls asymmetric localization of polarity proteins in pupal wing cells. PMID: 18804371
Results suggest that Flamingo mediates an interaction between sensory neuron growth cones and intermediate targets, which is required for continued axon advance. PMID: 19146847

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Subcellular Location

Cell membrane; Multi-pass membrane protein. Apical cell membrane.

Protein Families

G-protein coupled receptor 2 family

Tissue Specificity

In the pupal wing, expressed at relatively even levels in all regions. Abundant in 6-9 hours embryos. Expressed at higher levels in pupae than larvae.

Database Links

KEGG: dme:Dmel_CG11895

STRING: 7227.FBpp0303451

UniGene: Dm.4360

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stan Antibody

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