stpA Antibody

Datasheet

Code	CSB-PA364796XA01ENV
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Target Background

Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) stpA Polyclonal antibody

Uniprot No.

P0ACG1

Target Names

stpA

Alternative Names

stpA antibody; hnsB antibody; b2669 antibody; JW2644 antibody; DNA-binding protein StpA antibody; H-NS homolog StpA antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

A synthesized peptide derived from Escherichia coli (strain K12) stpA protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, Dot blot (guaranteed specific to the immunizing peptide)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

①. 100ug * KLH-conjugated peptide paired with 100ug * BSA-conjugated peptide (positive control);
②. 1ml*pre-immune serum (used as negative control) for free;
③. Rabbit polyclonal antibodies purified by Antigen Affinity;

Quality Guarantee

1. CUSABIO guarantees the antibody purity > 95% confirmed by SDS-PAGE.
2. CUSABIO guarantees the antibody titer > 1: 64,000 confirmed by ELISA.
3. CUSABIO guarantees to offer Peptide-specific Dot blot.
4. CUSABIO guarantees 100% risk-free: No qualified antibody, no charge.

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

A DNA-binding protein that acts in a fashion similar to H-NS protein upon overexpression, represses a number of genes including the cryptic blg operon, hns, papB and the proU locus. A subset of H-NS/StpA-regulated genes also require Hha for repression; Hha and Cnu (YdgT) increases the number of genes DNA bound by H-NS/StpA and may also modulate the oligomerization of the H-NS/StpA-complex. Repression can be inhibited by dominant-negative mutants of StpA or H-NS.; (Microbial infection) Originally isolated as a suppressor of a splicing defect of the thymidylate synthase (td) gene from bacteriophage T4. Acts as an RNA chaperone, accelerating splicing of viral pre-mRNA. Binds preferentially to unstructured over structured RNA; does not have a detectable high-affinity RNA-binding site in the pre-mRNA. There do not seem to be any specific RNA targets in transcribed E.coli DNA.

Gene References into Functions

In contrast to H-NS, the StpA filament interacts with a naked DNA segment to cause DNA bridging which results in simultaneous stiffening and bridging of DNA. PMID: 22187157
forms complexes with YdgT in vivo PMID: 15458420
The structural stability of an RNA determines whether the RNA chaperone activity of StpA is beneficial to folding. PMID: 15849314
StpA binding hinders DNA cleavage by both the non-specific endonuclease, DNase I, and by the site-specific type I restriction endonuclease, EcoKI. PMID: 16299353
Analysis of the relationship of stpA RNA chaperone activity to its RNA-binding properties. PMID: 17267410
StpA in homologous recombination and double-strand break repair is discussed PMID: 17991999

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Subcellular Location

Cytoplasm, nucleoid.

Protein Families

Histone-like protein H-NS family

Database Links

KEGG: ecj:JW2644

STRING: 316385.ECDH10B_2836

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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stpA Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background