stpA Antibody

Code CSB-PA364796XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) stpA Polyclonal antibody
Uniprot No.
Target Names
stpA
Alternative Names
stpA antibody; hnsB antibody; b2669 antibody; JW2644 antibody; DNA-binding protein StpA antibody; H-NS homolog StpA antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) stpA protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
A DNA-binding protein that acts in a fashion similar to H-NS protein upon overexpression, represses a number of genes including the cryptic blg operon, hns, papB and the proU locus. A subset of H-NS/StpA-regulated genes also require Hha for repression; Hha and Cnu (YdgT) increases the number of genes DNA bound by H-NS/StpA and may also modulate the oligomerization of the H-NS/StpA-complex. Repression can be inhibited by dominant-negative mutants of StpA or H-NS.; (Microbial infection) Originally isolated as a suppressor of a splicing defect of the thymidylate synthase (td) gene from bacteriophage T4. Acts as an RNA chaperone, accelerating splicing of viral pre-mRNA. Binds preferentially to unstructured over structured RNA; does not have a detectable high-affinity RNA-binding site in the pre-mRNA. There do not seem to be any specific RNA targets in transcribed E.coli DNA.
Gene References into Functions
  1. In contrast to H-NS, the StpA filament interacts with a naked DNA segment to cause DNA bridging which results in simultaneous stiffening and bridging of DNA. PMID: 22187157
  2. forms complexes with YdgT in vivo PMID: 15458420
  3. The structural stability of an RNA determines whether the RNA chaperone activity of StpA is beneficial to folding. PMID: 15849314
  4. StpA binding hinders DNA cleavage by both the non-specific endonuclease, DNase I, and by the site-specific type I restriction endonuclease, EcoKI. PMID: 16299353
  5. Analysis of the relationship of stpA RNA chaperone activity to its RNA-binding properties. PMID: 17267410
  6. StpA in homologous recombination and double-strand break repair is discussed PMID: 17991999

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Subcellular Location
Cytoplasm, nucleoid.
Protein Families
Histone-like protein H-NS family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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