Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) STT3 Polyclonal antibody

Uniprot No.

P39007

Target Names

STT3

Alternative Names

STT3 antibody; YGL022WDolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 antibody; Oligosaccharyl transferase subunit STT3 antibody; EC 2.4.99.18 antibody; Staurosporine and temperature sensitivity protein 3 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) STT3 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Catalytic subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. This subunit contains the active site and the acceptor peptide and donor lipid-linked oligosaccharide (LLO) binding pockets.

Gene References into Functions

results show that most of the STT3 TMDs are well inserted into the ER membrane on their own or in the presence of the natural flanking residues. PMID: 28512128
Eukaryotic N-glycosylation occurs via the membrane-anchored C-terminal domain of the Stt3p subunit of oligosaccharyltransferase. PMID: 22865878
The cloning, overexpression, purification, and biophysical characterization of the carboxyl-terminal domain of Stt3p is reported; Asp(518) plays a critical structural role, in addition to its previously proposed catalytic role. PMID: 20047336
is an essential player in N-glycosylation PMID: 15590627
results suggest that membrane protein STT3 has 11 transmembrane helices and an overall N(cyt)-C(lum) orientation PMID: 15781470
Homologous Stt3 proteins can serve analogous functions in distantly related eukaryotic cells regardless of their degree of conservation. PMID: 15936094

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Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Protein Families

STT3 family

Database Links

KEGG: sce:YGL022W

STRING: 4932.YGL022W

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STT3 Antibody

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