sub Antibody

Code CSB-PA895106XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) sub Polyclonal antibody
Uniprot No.
Target Names
sub
Alternative Names
sub antibody; Dub antibody; CG12298 antibody; Kinesin-like protein subito antibody; Protein double or nothing antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) sub protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required during female meiosis for bipolar spindle formation in the absence of the centrosomes and chromosome homolog segregation. Also has roles in male meiosis and mitotic divisions of the early embryo.
Gene References into Functions
  1. N- and C-terminal domains of Subito, while not required for localization to the central spindle microtubules, have important roles regulating Subito. PMID: 29514846
  2. Subito has undergone three single-gene transpositions and it is the nearest gene to the breakpoints of other single-gene transpositions and of two chromosomal inversions. PMID: 25135946
  3. Encodes a kinesin-like protein required for meiotic spindle pole formation in oocytes. PMID: 11973304
  4. Subito is required for establishing and/or maintaining the central spindle in Drosophila oocytes, and this substitutes for the role of centrosomes in organizing the bipolar spindle. PMID: 16055508
  5. Subito localizes to the spindle midzone at anaphase and is required for the localization of Polo, Incenp and Aurora B. PMID: 17077127

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Subcellular Location
Cytoplasm. Cytoplasm, cytoskeleton. Note=Microtubule-associated.
Protein Families
TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family
Database Links

KEGG: dme:Dmel_CG12298

STRING: 7227.FBpp0086041

UniGene: Dm.2051

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