SuUR Antibody

Code CSB-PA896146XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) SuUR Polyclonal antibody
Uniprot No.
Target Names
SuUR
Alternative Names
SuUR antibody; Su(UR)ES antibody; CG7869 antibody; Protein suppressor of underreplication antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) SuUR protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required for underreplication of DNA, which is found in many late replicating euchromatic regions of salivary gland polytene chromosomes. Functions by promoting the localization and retention of Rif1 to active DNA replication forks where Rif1 inhibits replication fork progression. Controls chromatin organization in polytene chromosomes.
Gene References into Functions
  1. Recruitment of either HP1(GAL4DBD) or SUUR(GAL4DBD) resulted in delayed completion of replication PMID: 25398563
  2. SUUR protein binding correlates with gene regulation and specific chromatin types. PMID: 24292500
  3. Our findings support the idea that the effect of SUUR on replication elongation depends on the cell cycle stage and can be mediated through its physical interaction with replication fork. PMID: 23149855
  4. Mutation of two conserved amino acid residues in this region impairs binding of SUUR to polytene chromosomes and reduces the ability of the protein to cause DNA underreplication. PMID: 19596903
  5. microdissection and sequence analysis of its pericentric heterochromatin PMID: 12111334
  6. SuUR mutation leads to earlier completion of DNA replication PMID: 12644953
  7. Differential responses of polyploid and diploid cells to ectopic SuUR may reflect differences in the mechanisms underlying mitotic cell cycles and endocycles PMID: 14508681
  8. SuUR binds specificialy to boundary regions and regular distribution of boundary regions in chromosomes may represent the dispersion of sites designed for chromosomal silencing PMID: 14702350
  9. SUUR has at least two separate chromosome-binding regions that are able to recognize intercalary and pericentric heterochromatin specifically. PMID: 16134332
  10. The SuUR gene affects the distribution of HP1 and SU(VAR)3-9 on the nurse cell poytene chromosomes. PMID: 16607511
  11. Data on the SuUR gene, its expression, and effect on polytene chromosome structure and replication are summarized. PMID: 17025152
  12. these results suggest that SUUR is a ubiquitous marker of heterochromatin in different cell types PMID: 17606990
  13. describe how, in double mutants for Suppressor of variegation 3-9 and SuUR genes encoding two structural proteins of heterochromatin, new banded heterochromatic segments appear in all polytene chromosomes PMID: 17640911
  14. Reversible decondensation of heterochromatin regions of polytene chromosomes during ectopic expression of the SuUR gene. PMID: 19650328
  15. Suppressor of Underreplication gene does not directely affect the replication progression PMID: 19817130

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Subcellular Location
Nucleus. Chromosome.
Tissue Specificity
Expressed throughout development. Weakly expressed. Expressed at higher level in embryos and adult females.
Database Links

KEGG: dme:Dmel_CG7869

STRING: 7227.FBpp0075933

UniGene: Dm.7710

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