Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SUVH4 Polyclonal antibody

Uniprot No.

Q8GZB6

Target Names

SUVH4

Alternative Names

SUVH4 antibody; KYP antibody; SDG33 antibody; SET33 antibody; At5g13960 antibody; MAC12.7 antibody; Histone-lysine N-methyltransferase antibody; H3 lysine-9 specific SUVH4 antibody; EC 2.1.1.- antibody; Histone H3-K9 methyltransferase 4 antibody; H3-K9-HMTase 4 antibody; Protein KRYPTONITE antibody; Protein SET DOMAIN GROUP 33 antibody; Suppressor of variegation 3-9 homolog protein 4 antibody; Su(var)3-9 homolog protein 4 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana SUVH4 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Histone methyltransferase. Methylates 'Lys-9' of histone H3. H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression. The silencing mechanism via DNA CpNpG methylation requires the targeting of chromomethylase CMT3 to methylated histones, probably through an interaction with an HP1-like adapter. By its function, KYP is directly required for the maintenance of the DNA CpNpG and asymmetric methylation. Involved in the silencing of transposable elements.

Gene References into Functions

CMT3-SUVH4 is the major pathway controlling silencing of the exonic Evelknievel retroelement via inducing non-CG methylation. PMID: 30446008
The authors conclude that geminivirus TrAP attenuates the transcriptional gene silencing of the viral chromatin by inhibiting KYP activity to evade host surveillance. PMID: 26344546
The structures of KYP and previously reported CMT3 complexes provide insights into molecular mechanisms linking DNA and histone methylation. PMID: 25018018
Genetic analysis showed that DOG1 and HUB1 are epistatic to KYP/SUVH4, suggesting that these genes regulate seed dormancy in the same genetic pathway. PMID: 22122546
H3 lysine 9 methylation epigenetic modifications are reduced in an suvh4 suvh6 double mutant PMID: 16287862

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Subcellular Location

Nucleus. Chromosome. Chromosome, centromere. Note=Associates with centromeric constitutive heterochromatin and at a lower level with regions of euchromatin.

Protein Families

Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, Suvar3-9 subfamily

Tissue Specificity

Expressed in leaves stems and flowers.

Database Links

KEGG: ath:AT5G13960

STRING: 3702.AT5G13960.1

UniGene: At.8330

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SUVH4 Antibody

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