Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SWA2 Polyclonal antibody

Uniprot No.

Q06677

Target Names

SWA2

Alternative Names

SWA2 antibody; AUX1 antibody; BUD24 antibody; YDR320C antibody; D9798.10 antibody; Auxilin-like clathrin uncoating factor SWA2 antibody; Bud site selection protein 24 antibody; DnaJ-related protein SWA2 antibody; J protein SWA2 antibody; Synthetic lethal with ARF1 protein 2 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SWA2 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Cofactor for the uncoating of clathrin-coated vesicles (CCVs) by Hsp70-type chaperones (SSA1/2/3 and SSB1/2). Coat disassembly is important for fusion of vesicles with target membranes and for recycling components of clathrin coats to the cytoplasm for further rounds of vesicle formation. Binds to assembled clathrin and recruits the ATP-activated chaperone to CCVs. Stimulates the ATPase activity of the clathrin-associated Hsp70-type chaperone SSA1, which then disrupts clathrin-clathrin interactions, leading to release of the clathrin coat. In addition, prevents unproductive clathrin assembly in the cell. Also required for cortical endoplasmic reticulum inheritance.

Gene References into Functions

These investigations suggest that Hsp90 may be the critical binding partner of Swa2 in [URE3] propagation, and we propose a new model for plausible Swa2-Hsp90 cooperation in [URE3] prion propagation. PMID: 28574745
study reports novel role for Swa2/Aux1; found that, in addition to Sis1,[URE3-1] is specifically dependent upon Swa2; data suggest that prion-chaperone specificity arises from the Swa2 TPR domain and supports a model where Swa2 acts through Hsp70, most likely to provide additional access points for Hsp104 to promote prion template generation PMID: 26031938
Both the TPR and J-domains of Swa2p, but not its clathrin-binding and ubiquitin-association motifs, are required for its function in Flo11p processing. PMID: 20138883
These data suggest that the TPR and J-domains of SWA2 collaborate in a bipartite interaction with Hsp70 to regulate its activity in clathrin disassembly. PMID: 16687570
Ubiquitin (Ub) recognition occurs predominantly through an atypical interaction in which the ubiquitin-associated (UBA) helix alpha1 and the N-terminal part of helix alpha2 of Swa2 bind to Ub. PMID: 18948116

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Subcellular Location

Cytoplasm. Endoplasmic reticulum membrane; Peripheral membrane protein.

Database Links

KEGG: sce:YDR320C

STRING: 4932.YDR320C

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SWA2 Antibody

Product Details

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Target Background