SWC6 Antibody

Code CSB-PA780841XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SWC6 Polyclonal antibody
Uniprot No.
Target Names
SWC6
Alternative Names
SWC6 antibody; SEF antibody; At5g37055 antibody; MJG14.20 antibody; MJG14.4 antibody; SWR1 complex subunit 6 antibody; Protein SERRATED LEAVES AND EARLY FLOWERING antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana SWC6 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant H2A.F/Z leading to transcriptional regulation of selected genes (e.g. FLC) by chromatin remodeling. Coodinates SWR1-C, FRI-C (FLC transcription activator complex), histone methyltransferase and general transcription factors. Represses flowering by positively regulating FLC and MAF4. Binds to the promoter region of FLC chromatin.
Gene References into Functions
  1. SKIP is required for the splicing of serrated leaves and early flowering (SEF) pre-messenger RNA (mRNA), which encodes a component of the ATP-dependent SWR1 chromatin remodeling complex (SWR1-C). PMID: 28893254
  2. SERRATED LEAVES AND EARLY FLOWERING (SEF) gene represses flowering at least in part by positively regulating the expression of the FLC and MAF4 genes in A. thaliana. It interacts with ATARP6 and PIE1 to form a molecular complex. [SEF] PMID: 17142478
  3. Arabidopsis SWC6 (AtSWC6), SUPPRESSOR OF FRIGIDA 3 (SUF3) and PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 (PIE1) are homologs of SWC6, ARP6 and SWR1 with roles in development, including floral repression through activation of FLOWERING LOCUS C [SWC6] PMID: 17470967
  4. part of an Arabidopsis SWR1 chromatin remodelling complex involved in the regulation of diverse aspects of plant development, including floral repression through the activation of FLC and FLC-like genes PMID: 18296430
Subcellular Location
Nucleus speckle. Nucleus. Note=Relocates to the nuclear periphery when interacting with ARP6.
Protein Families
ZNHIT1 family
Tissue Specificity
Expressed in root, lateral root primordia, shoot apex, leaves, stems, inflorescences, flowers, axillary buds, developing siliques and premature seeds.
Database Links

KEGG: ath:AT5G37055

STRING: 3702.AT5G37055.1

UniGene: At.9468

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