Product Details

Full Product Name

Rabbit anti-Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) swi1 Polyclonal antibody

Uniprot No.

Q9UUM2

Target Names

swi1

Alternative Names

swi1 antibody; SPBC216.06c antibody; Mating-type switching protein swi1 antibody; Replication fork protection complex subunit swi1 antibody

Raised in

Rabbit

Species Reactivity

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Immunogen

Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) swi1 protein

Immunogen Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Forms a fork protection complex (FPC) with swi3. FPC coordinates leading and lagging strand synthesis and moves with the replication fork. It is required for programmed fork-pausing which is necessary for mating-type switching. FPC stabilizes replication forks in a configuration that is recognized by replication checkpoint sensors. It is involved in termination at the mat1-proximal polar-terminator of replication (RTS1) and also required for activation of the Rad53-like checkpoint kinase cds1.

Gene References into Functions

These results suggest that Swi1 ensures telomere replication by suppressing recombination and repeat instability at telomeres. Our studies may also be relevant in understanding the potential role of Swi1 in regulation of telomere stability in cancer cells PMID: 26990647
Swi1 associates with chromatin through its DDT domain and recruits Swi3 to function together as the replication fork protection complex PMID: 22952839
Fission yeast Swi1-Swi3 complex facilitates DNA binding of Mrc1. PMID: 20924116
Results support the notion that Swi1p and Swi3p act at several different protein-DNA complexes in the rDNA spacer regions to arrest replication but that not all fork barriers require their activity to arrest forks. [swi1] PMID: 15371597
RFC(Ctf18) and the Swi1-Swi3 complex function in separate and redundant pathways essential for replication fork stabilization to facilitate sister chromatid cohesion in fission yeast. PMID: 18045993
Swi1, the TIMELESS homologue, differentially controls the recombination potential of replication fork barriers, switching between being a suppressor and an activator of recombination in a site-specific fashion. PMID: 19273851

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Subcellular Location

Nucleus. Note=Associated with chromatin during S phase. Nuclear accumulation requires swi3.

Database Links

KEGG: spo:SPBC216.06c

STRING: 4896.SPBC216.06c.1

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swi1 Antibody

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Target Background