Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SYD Polyclonal antibody

Uniprot No.

F4IHS2

Target Names

SYD

Alternative Names

SYD antibody; CHR3 antibody; At2g28290 antibody; T3B23 antibody; Chromatin structure-remodeling complex protein SYD antibody; EC 3.6.4.- antibody; ATP-dependent helicase SYD antibody; Protein CHROMATIN REMODELING 3 antibody; Protein SPLAYED antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana SYD protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Catalytic component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. Controls stem cell fate via the transcription regulation of WUS in the shoot apical meristem, by modulating its promoter. LFY-dependent repressor of the meristem identity switch from vegetative to reproductive development probably by modulating chromatin state. Involved in the regulation of floral homeotic gene expression in response to environmental stimuli. Required for carpel and ovule development, and for cotyledon separation via the regulation of CUC2 transcription. Regulates the promoters of several genes downstream of the jasmonate (JA) and ethylene (ET) signaling pathways. Required for resistance against the necrotrophic pathogen B.cinerea but not the biotrophic pathogen P.syringae.

Gene References into Functions

SYD plays a subtle, specific role in the regulation of SNC1 expression and SNC1-mediated immunity. SYD may work with other proteins at the chromatin level to repress SNC1 transcription. PMID: 26063389
The SWI2/SNF2 chromatin-remodeling ATPases SPLAYED (SYD) and BRAHMA (BRM) are redundantly required for flower patterning and for the activation of AP3 and AG. PMID: 22323601
SYD is recruited to the WUS promoter and is involved in regulation of the stem cell pool maintenance via direct transcriptional control of this master regulator. PMID: 15833920
The N-terminal ATPase AT-hook-containing region of the SYD protein is responsible for its biological activity. PMID: 16640604
Results describe overlapping functions for the two SWI/SNF ATPases, SPLAYED and BRAHMA, and indicate that these proteins exhibit remarkable regulatory specificity. PMID: 17293567
study found that the chromatin remodeling protein, SPLAYED, is required for gene expression within specific biotic stress signaling networks PMID: 19079584

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Subcellular Location

Cytoplasm. Nucleus.

Protein Families

SNF2/RAD54 helicase family

Tissue Specificity

Mostly expressed in rapidly dividing cells in the vegetative, inflorescence, and root meristems, as well as in young leaf and flower primordia. Isoform 1 is predominantly found in seedlings whereas isoform 2 is present in both seedlings and inflorescences

Database Links

KEGG: ath:AT2G28290

STRING: 3702.AT2G28290.1

UniGene: At.22414

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SYD Antibody

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